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Signal Transduction Signaling Pathway Nuclear Signaling NFkB Pathway
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Anti-IkB beta (phospho S23) antibody (ab75746)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IkB beta (phospho S23) antibody (ab75746)

    Key features and details

    • Rabbit polyclonal to IkB beta (phospho S23)
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Recombinant Human IKB beta protein (ab85246)

    View more associated products

    Overview

    • Product name

      Anti-IkB beta (phospho S23) antibody
      See all IKB beta primary antibodies
    • Description

      Rabbit polyclonal to IkB beta (phospho S23)
    • Host species

      Rabbit
    • Specificity

      ab75746 detects endogenous levels of total IkB beta protein.
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Synthetic phosphopeptide derived from human IkB beta around the phosphorylation site of serine 23 (L-G-SP-L-G ).

    • Positive control

      • Human breast carcinoma tissue.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS

      Without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab75746 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • NFkB Pathway
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • NFkB pathway
      • Immunology
      • Innate Immunity
      • TLR Signaling

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human IKB beta protein (ab85246)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab75746 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P
    1/50 - 1/100.
    Notes
    IHC-P
    1/50 - 1/100.

    Target

    • Function

      Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
    • Tissue specificity

      Expressed in all tissues examined.
    • Sequence similarities

      Belongs to the NF-kappa-B inhibitor family.
      Contains 6 ANK repeats.
    • Post-translational
      modifications

      Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession Q15653 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4793 Human
      • Omim: 604495 Human
      • SwissProt: Q15653 Human
      • Unigene: 9731 Human
      • Alternative names

        • I kappa B beta antibody
        • I-kappa-B-beta antibody
        • IkappaBbeta antibody
        • IKB beta antibody
        • IkB-B antibody
        • IkB-beta antibody
        • IKBB antibody
        • IKBB_HUMAN antibody
        • IkBbeta antibody
        • NF kappa BIB antibody
        • NF-kappa-B inhibitor beta antibody
        • NF-kappa-BIB antibody
        • Nfkbib antibody
        • Thyroid receptor interacting protein 9 antibody
        • Thyroid receptor-interacting protein 9 antibody
        • TR interacting protein 9 antibody
        • TR-interacting protein 9 antibody
        • TRIP-9 antibody
        • TRIP9 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IkB beta (phospho S23) antibody (ab75746)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IkB beta (phospho S23) antibody (ab75746)
        ab75746, at a 1/50 dilution, staining IkB beta in paraffin embedded human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunizing phospho peptide.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab75746? Please let us know so that we can cite the reference in this datasheet.

      ab75746 has been referenced in 2 publications.

      • Zhou ZX  et al. Anti-inflammatory activity of resveratrol prevents inflammation by inhibiting NF-?B in animal models of acute pharyngitis. Mol Med Rep 17:1269-1274 (2018). PubMed: 29115472
      • Kane J  et al. Hematopoietic stem and progenitor cell migration after hypofractionated radiation therapy in a murine model. Int J Radiat Oncol Biol Phys 87:1162-70 (2013). Mouse . PubMed: 24113056

      Customer reviews and Q&As

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