Anti-IkB beta (phospho S23) antibody (ab75746)
Key features and details
- Rabbit polyclonal to IkB beta (phospho S23)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-IkB beta (phospho S23) antibody
See all IKB beta primary antibodies -
Description
Rabbit polyclonal to IkB beta (phospho S23) -
Host species
Rabbit -
Specificity
ab75746 detects endogenous levels of total IkB beta protein. -
Tested Applications & Species
Application Species IHC-P Human -
Immunogen
Synthetic phosphopeptide derived from human IkB beta around the phosphorylation site of serine 23 (L-G-SP-L-G ).
-
Positive control
- Human breast carcinoma tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab75746 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
IHC-P |
Human
|
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100.
|
Notes |
---|
IHC-P
1/50 - 1/100. |
Target
-
Function
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation. -
Tissue specificity
Expressed in all tissues examined. -
Sequence similarities
Belongs to the NF-kappa-B inhibitor family.
Contains 6 ANK repeats. -
Post-translational
modificationsPhosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 4793 Human
- Omim: 604495 Human
- SwissProt: Q15653 Human
- Unigene: 9731 Human
-
Alternative names
- I kappa B beta antibody
- I-kappa-B-beta antibody
- IkappaBbeta antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IkB beta (phospho S23) antibody (ab75746)ab75746, at a 1/50 dilution, staining IkB beta in paraffin embedded human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunizing phospho peptide.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab75746 has been referenced in 2 publications.
- Zhou ZX et al. Anti-inflammatory activity of resveratrol prevents inflammation by inhibiting NF-?B in animal models of acute pharyngitis. Mol Med Rep 17:1269-1274 (2018). PubMed: 29115472
- Kane J et al. Hematopoietic stem and progenitor cell migration after hypofractionated radiation therapy in a murine model. Int J Radiat Oncol Biol Phys 87:1162-70 (2013). Mouse . PubMed: 24113056