Anti-IkB beta (phospho S23) antibody (ab75746)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-IkB beta (phospho S23) antibody
See all IKB beta primary antibodies -
DescriptionRabbit polyclonal to IkB beta (phospho S23)
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Host speciesRabbit
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Specificityab75746 detects endogenous levels of total IkB beta protein.
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Tested applicationsSuitable for: IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic phosphopeptide derived from human IkB beta around the phosphorylation site of serine 23 (L-G-SP-L-G ).
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Positive control
- Human breast carcinoma tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab75746 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab75746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. |
Target
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FunctionInhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
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Tissue specificityExpressed in all tissues examined.
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Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
Contains 6 ANK repeats. -
Post-translational
modificationsPhosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 4793 Human
- Entrez Gene: 18036 Mouse
- Entrez Gene: 81525 Rat
- Omim: 604495 Human
- SwissProt: Q15653 Human
- SwissProt: Q60778 Mouse
- SwissProt: Q9JIA3 Rat
- Unigene: 9731 Human
see all -
Alternative names
- I kappa B beta antibody
- I-kappa-B-beta antibody
- IkappaBbeta antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IkB beta (phospho S23) antibody (ab75746)ab75746, at a 1/50 dilution, staining IkB beta in paraffin embedded human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunizing phospho peptide.
Protocols
References
This product has been referenced in:
- Kane J et al. Hematopoietic stem and progenitor cell migration after hypofractionated radiation therapy in a murine model. Int J Radiat Oncol Biol Phys 87:1162-70 (2013). Mouse . Read more (PubMed: 24113056) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"