Key features and details
- Rabbit polyclonal to IkB beta (phospho S23)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IkB beta (phospho S23) antibody
See all IKB beta primary antibodies
DescriptionRabbit polyclonal to IkB beta (phospho S23)
Specificityab75746 detects endogenous levels of total IkB beta protein.
Tested Applications & Species
Application Species IHC-PHuman
Synthetic phosphopeptide derived from human IkB beta around the phosphorylation site of serine 23 (L-G-SP-L-G ).
- Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75746 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
1/50 - 1/100.
1/50 - 1/100.
FunctionInhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
Tissue specificityExpressed in all tissues examined.
Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
Contains 6 ANK repeats.
modificationsPhosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- I kappa B beta antibody
- I-kappa-B-beta antibody
- IkappaBbeta antibody
ab75746 has been referenced in 2 publications.
- Zhou ZX et al. Anti-inflammatory activity of resveratrol prevents inflammation by inhibiting NF-?B in animal models of acute pharyngitis. Mol Med Rep 17:1269-1274 (2018). PubMed: 29115472
- Kane J et al. Hematopoietic stem and progenitor cell migration after hypofractionated radiation therapy in a murine model. Int J Radiat Oncol Biol Phys 87:1162-70 (2013). Mouse . PubMed: 24113056