Product nameAnti-IKB epsilon antibody
See all IKB epsilon primary antibodies
DescriptionRabbit polyclonal to IKB epsilon
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow
Synthetic peptide corresponding to Human IKB epsilon (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: O00221
- WB: Jurkat, HeLa and PC-12 whole cell lysates. IHC-P: Human heart tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 53 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
FunctionInhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Tissue specificityHighly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
Contains 6 ANK repeats.
modificationsSerine phosphorylated; followed by proteasome-dependent degradation.
- Information by UniProt
- I kappa B epsilon antibody
- I-kappa-B-epsilon antibody
- IkappaBepsilon antibody
All lanes : Anti-IKB epsilon antibody (ab230901) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 53 kDa
Formalin-fixed, paraffin-embedded human heart tissue stained for IKB epsilon with ab230901 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for IKB epsilon (red) using ab230901 at 1/100 dilution followed by DyLight 594-conjugated secondary antibody in ICC/IF.
The nuclear counter stain is DAPI (blue).
ab230901 has not yet been referenced specifically in any publications.