Key features and details
- Rabbit polyclonal to IKB epsilon
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-IKB epsilon antibody
See all IKB epsilon primary antibodies
DescriptionRabbit polyclonal to IKB epsilon
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow
Synthetic peptide corresponding to Human IKB epsilon (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: O00221
- WB: Jurkat, HeLa and PC-12 whole cell lysates. IHC-P: Human heart tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 53 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
FunctionInhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Tissue specificityHighly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
Contains 6 ANK repeats.
modificationsSerine phosphorylated; followed by proteasome-dependent degradation.
- Information by UniProt
- I kappa B epsilon antibody
- I-kappa-B-epsilon antibody
- IkappaBepsilon antibody
All lanes : Anti-IKB epsilon antibody (ab230901) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 53 kDa
Formalin-fixed, paraffin-embedded human heart tissue stained for IKB epsilon with ab230901 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for IKB epsilon (red) using ab230901 at 1/100 dilution followed by DyLight 594-conjugated secondary antibody in ICC/IF.
The nuclear counter stain is DAPI (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230901 has not yet been referenced specifically in any publications.