Overview

  • Product name

  • Description

    Rabbit polyclonal to IKB epsilon
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide corresponding to Human IKB epsilon (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: O00221

  • Positive control

    • WB: Jurkat, HeLa and PC-12 whole cell lysates. IHC-P: Human heart tissue. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 53 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
  • Tissue specificity

    Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
  • Sequence similarities

    Belongs to the NF-kappa-B inhibitor family.
    Contains 6 ANK repeats.
  • Post-translational
    modifications

    Serine phosphorylated; followed by proteasome-dependent degradation.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • I kappa B epsilon antibody
    • I-kappa-B-epsilon antibody
    • IkappaBepsilon antibody
    • IkB E antibody
    • IkB-E antibody
    • IkB-epsilon antibody
    • IKBE antibody
    • IKBE_HUMAN antibody
    • MGC72568 antibody
    • NF kappa B inhibitor epsilon antibody
    • NF kappa BIE antibody
    • NF-kappa-B inhibitor epsilon antibody
    • NF-kappa-BIE antibody
    • NFkappa BIE antibody
    • NFkappaB inhibitor epsilon antibody
    • NFKBIE antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon antibody
    • OTTHUMP00000016522 antibody
    • Slc35b2 antibody
    • solute carrier family 35, member B2 antibody
    see all

Images

  • All lanes : Anti-IKB epsilon antibody (ab230901) at 1/500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 53 kDa

  • Formalin-fixed, paraffin-embedded human heart tissue stained for IKB epsilon with ab230901 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for IKB epsilon (red) using ab230901 at 1/100 dilution followed by DyLight 594-conjugated secondary antibody in ICC/IF. 

    The nuclear counter stain is DAPI (blue).

References

ab230901 has not yet been referenced specifically in any publications.

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