Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR901(2)] to IKB epsilon
- Suitable for: WB, IP, IHC-P, ICC
- Reacts with: Human
Product nameAnti-IKB epsilon antibody [EPR901(2)]
See all IKB epsilon primary antibodies
DescriptionRabbit monoclonal [EPR901(2)] to IKB epsilon
Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human IKB epsilon. (Uniprot: O00221)
- THP-1, Jurkat, HeLa and A375 cell lysates; Human tonsil tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 53 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
FunctionInhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Tissue specificityHighly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
Contains 6 ANK repeats.
modificationsSerine phosphorylated; followed by proteasome-dependent degradation.
- Information by UniProt
- I kappa B epsilon antibody
- I-kappa-B-epsilon antibody
- IkappaBepsilon antibody
All lanes : Anti-IKB epsilon antibody [EPR901(2)] (ab134143) at 1/1000 dilution
Lane 1 : THP-1 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of Paraffin-embedded Human tonsil tissue labelling IKB epsilon with ab134143 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab134143 has been referenced in 1 publication.
- Chu LY et al. Cytokine-induced autophagy promotes long-term VCAM-1 but not ICAM-1 expression by degrading late-phase I?Ba. Sci Rep 7:12472 (2017). PubMed: 28963466