Overview

  • Product name

    Anti-IKB epsilon antibody [EPR901(2)]
    See all IKB epsilon primary antibodies
  • Description

    Rabbit monoclonal [EPR901(2)] to IKB epsilon
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human IKB epsilon. (Uniprot: O00221)

  • Positive control

    • THP-1, Jurkat, HeLa and A375 cell lysates; Human tonsil tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 53 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
    • Tissue specificity

      Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
    • Sequence similarities

      Belongs to the NF-kappa-B inhibitor family.
      Contains 6 ANK repeats.
    • Post-translational
      modifications

      Serine phosphorylated; followed by proteasome-dependent degradation.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • I kappa B epsilon antibody
      • I-kappa-B-epsilon antibody
      • IkappaBepsilon antibody
      • IkB E antibody
      • IkB-E antibody
      • IkB-epsilon antibody
      • IKBE antibody
      • IKBE_HUMAN antibody
      • MGC72568 antibody
      • NF kappa B inhibitor epsilon antibody
      • NF kappa BIE antibody
      • NF-kappa-B inhibitor epsilon antibody
      • NF-kappa-BIE antibody
      • NFkappa BIE antibody
      • NFkappaB inhibitor epsilon antibody
      • NFKBIE antibody
      • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon antibody
      • OTTHUMP00000016522 antibody
      • Slc35b2 antibody
      • solute carrier family 35, member B2 antibody
      see all

    Images

    • All lanes : Anti-IKB epsilon antibody [EPR901(2)] (ab134143) at 1/1000 dilution

      Lane 1 : THP-1 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 53 kDa
      Observed band size: 45 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of Paraffin-embedded Human tonsil tissue labelling IKB epsilon with ab134143 at 1/100 dilution.

    References

    This product has been referenced in:

    • Chu LY  et al. Cytokine-induced autophagy promotes long-term VCAM-1 but not ICAM-1 expression by degrading late-phase I?Ba. Sci Rep 7:12472 (2017). Read more (PubMed: 28963466) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134143.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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