Key features and details
- Rabbit polyclonal to IKB epsilon (phospho S22)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-IKB epsilon (phospho S22) antibody
See all IKB epsilon primary antibodies
DescriptionRabbit polyclonal to IKB epsilon (phospho S22)
Specificityab75907 detects endogenous levels of IKB epsilon only when phosphorylated at serine 22.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic phosphopeptide derived from human IKB epsilon around the phosphorylation site of serine 22 (I-E-SP-L-R).
- Human breast carcinoma tissue; 293 cell extracts treated with TNF-alpha (20ng/ml, 15min).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75907 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab75907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 38 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionInhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Tissue specificityHighly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
Contains 6 ANK repeats.
modificationsSerine phosphorylated; followed by proteasome-dependent degradation.
- Information by UniProt
- I kappa B epsilon antibody
- I-kappa-B-epsilon antibody
- IkappaBepsilon antibody
ab75907 at 1/50 dilution staining IKB epsilon in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising peptide.
All lanes : Anti-IKB epsilon (phospho S22) antibody (ab75907) at 1/500 dilution
Lane 1 : 293 cell extracts, untreated
Lane 2 : 293 cell extracts, treated with TNF-alpha (20ng/ml, 15min)
Predicted band size: 38 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
ab75907 has not yet been referenced specifically in any publications.