Overview

  • Product name

  • Description

    Rabbit polyclonal to IKIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human IKIP aa 121-350.
    Database link: Q70UQ0

  • Positive control

    • WB: Mouse kidney lysate; HL-60 cell lysate. IHC-P: Human small intestine and tonsil tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab224674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 36, 39 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/20 - 1/200.

Target

  • Relevance

    IKIP (I kappa B kinase interacting protein)is located on chromosome 12 in close proximity to APAF1 (apoptotic protease-activating factor-1). IKIP was originally cloned from a yeast two hybrid screening as an IKK2 interacting protein. IKIP and APAF1 share a common 488 bp promoter from which the two genes are transcribed in opposite directions, presumably in a co-regulated manner. Three IKIP transcripts are generated by differential splicing and alternative exon usage and show no significant homology to other genes. IKIP is expressed predominantly in the lung, kidney, spleen, thymus and skeletal muscle. Like APAF1, expression of IKIP is enhanced by ?-irradiation, in a p53-dependent manner. When transfected into endothelial cells IKIP promotes apoptosis suggesting that it plays a role in the cell death process as well as NF-?B regulation.
  • Database links

  • Alternative names

    • FLJ31051 antibody
    • I kappa B kinase interacting protein antibody
    • IKBIP antibody
    • IKBKB interacting protein antibody
    • IKIP1 antibody
    • IKIP2 antibody
    • IKIP3 antibody
    • IKK interacting protein antibody
    • Inhibitor of nuclear factor kappa B kinase interacting protein antibody
    see all

Images

  • All lanes : Anti-IKIP antibody (ab224674) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : HL-60 (human promyelocytic leukemia cell line) cell lysate

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Predicted band size: 39 kDa
    Observed band size: 36,39 kDa
    why is the actual band size different from the predicted?

  • Paraffin-embedded human small intestine tissue stained for IKIP using ab224674 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human tonsil tissue stained for IKIP using ab224674 at 1/100 dilution in immunohistochemical analysis.

References

ab224674 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
U2OS cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 27 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
MEF cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 26 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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