Key features and details
- Rabbit polyclonal to IKIP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-IKIP antibody
See all IKIP primary antibodies
DescriptionRabbit polyclonal to IKIP
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human IKIP aa 121-350.
Database link: Q70UQ0
- WB: Mouse kidney lysate; HL-60 cell lysate. IHC-P: Human small intestine and tonsil tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 36, 39 kDa (predicted molecular weight: 39 kDa).|
|IHC-P||1/20 - 1/200.|
RelevanceIKIP (I kappa B kinase interacting protein)is located on chromosome 12 in close proximity to APAF1 (apoptotic protease-activating factor-1). IKIP was originally cloned from a yeast two hybrid screening as an IKK2 interacting protein. IKIP and APAF1 share a common 488 bp promoter from which the two genes are transcribed in opposite directions, presumably in a co-regulated manner. Three IKIP transcripts are generated by differential splicing and alternative exon usage and show no significant homology to other genes. IKIP is expressed predominantly in the lung, kidney, spleen, thymus and skeletal muscle. Like APAF1, expression of IKIP is enhanced by ?-irradiation, in a p53-dependent manner. When transfected into endothelial cells IKIP promotes apoptosis suggesting that it plays a role in the cell death process as well as NF-?B regulation.
- FLJ31051 antibody
- I kappa B kinase interacting protein antibody
- IKBIP antibody
All lanes : Anti-IKIP antibody (ab224674) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : HL-60 (human promyelocytic leukemia cell line) cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 36,39 kDa why is the actual band size different from the predicted?
Paraffin-embedded human small intestine tissue stained for IKIP using ab224674 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human tonsil tissue stained for IKIP using ab224674 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224674 has not yet been referenced specifically in any publications.