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    ikk-alpha--ikk-beta-antibody-epr16628-ab178870.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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RecombinantRabMAb

Recombinant Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (5) Submit a question References (40)

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Immunocytochemistry/ Immunofluorescence - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Flow Cytometry (Intracellular) - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Immunoprecipitation - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Immunoprecipitation - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Immunocytochemistry/ Immunofluorescence - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Immunoprecipitation - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
  • Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16628] to IKK alpha + IKK beta
  • Suitable for: IP, ICC/IF, WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-IKK alpha + IKK beta antibody [EPR16628]
    See all IKK alpha + IKK beta primary antibodies
  • Description

    Rabbit monoclonal [EPR16628] to IKK alpha + IKK beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC/IF, WB, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Daudi, HL-60, A431, 293T, C6, Raw264.7 and NIH/3T3 whole cell lysates. Human fetal kidney lysates. Mouse brain, Mouse kidney, Mouse spleen and Rat kidney lysates. ICC/IF: HeLa cells. IP: Jurkat whole cell extract.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16628
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-IKK alpha + IKK beta antibody [EPR16628] (ab200529)
    • Alexa Fluor® 488 Anti-IKK alpha + IKK beta antibody [EPR16628] (ab200618)
    • Anti-IKK alpha + IKK beta antibody [EPR16628] - BSA and Azide free (ab240210)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/40.
ICC/IF (4)
1/50.
WB (1)
1/1000. Detects a band of approximately 75, 87 kDa (predicted molecular weight: 85, 87 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
IP
1/40.
ICC/IF
1/50.
WB
1/1000. Detects a band of approximately 75, 87 kDa (predicted molecular weight: 85, 87 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.

Target

  • Relevance

    IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta). IKK alpha and IKK beta interact with each other and both are essential for NFkB activation.
  • Cellular localization

    Cytoplasmic and Nuclear; Membrane raft. Note: IKK beta is colocalized with DPP4 in membrane rafts.
  • Database links

    • Entrez Gene: 1147 Human
    • Entrez Gene: 3551 Human
    • Entrez Gene: 12675 Mouse
    • Entrez Gene: 16150 Mouse
    • Omim: 600664 Human
    • Omim: 603258 Human
    • SwissProt: O14920 Human
    • SwissProt: O15111 Human
    • SwissProt: O88351 Mouse
    • SwissProt: Q60680 Mouse
    • SwissProt: Q9QY78 Rat
    see all
  • Alternative names

    • CHUK antibody
    • Conserved helix loop helix ubiquitous kinase antibody
    • Conserved helix-loop-helix ubiquitous kinase antibody
    • I kappa B kinase 1 antibody
    • I kappa B kinase 2 antibody
    • I Kappa B kinase alpha antibody
    • I Kappa B kinase beta antibody
    • IkB kinase alpha subunit antibody
    • IkBKA antibody
    • IkBKB antibody
    • IKK a kinase antibody
    • IKK alpha antibody
    • IKK beta antibody
    • IKK-alpha antibody
    • IKK-beta antibody
    • IKK1 antibody
    • IKK2 antibody
    • IKKA antibody
    • IKKB antibody
    • IMD15 antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
    • NFKBIKA antibody
    • NFKBIKB antibody
    • Nuclear factor NF kappa B inhibitor kinase beta antibody
    • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
    • Nuclear factor NFkappaB inhibitor kinase alpha antibody
    • TCF 16 antibody
    • TCF-16 antibody
    • TCF16 antibody
    • Transcription factor 16 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Immunocytochemistry/ Immunofluorescence - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling IKK alpha + IKK beta with ab178870 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody (ab150077) at 1/200 dilution (green). Nuclear and cytoplasm staining is detected. 

    Tubulin is detected with Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). The nuclear counter stain is DAPI (blue). 

     

    The two negative controls were :-

    -ve control 1 - ab178870 at 1/50 diltuion followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.
    -ve control 2 - Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution.

  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    All lanes : Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysates
    Lane 3 : HL-60 (Human promyelocytic leukemia cells) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 85, 87 kDa
    Observed band size: 75,87 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

     

    ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.

  • Flow Cytometry (Intracellular) - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Flow Cytometry (Intracellular) - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IKK alpha + IKK beta with purified ab178870 at 1/130 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. 

     

     

  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    All lanes : Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution

    Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysates
    Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 85, 87 kDa
    Observed band size: 75,87 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.


    ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.

  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution + Human fetal kidney lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 85, 87 kDa
    Observed band size: 87 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    All lanes : Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870) at 1/1000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse kidney lysates
    Lane 3 : Mouse spleen lysates
    Lane 4 : Rat kidney lysates
    Lane 5 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 85, 87 kDa
    Observed band size: 75,87 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.


    ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.

  • Immunoprecipitation - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Immunoprecipitation - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)

    Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using ab178870 at 1/40 dilution. Western blot detection was performed using ab178870 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST. 

    ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.

     

     

  • Immunoprecipitation - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Immunoprecipitation - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)

    Cross-Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using ab178870 at 1/40 dilution. Western blot detection was performed using ab32041 (IKK alpha) at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Immunocytochemistry/ Immunofluorescence - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)

    This immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells is a comparison between ab178870 and a competitor’s leading rabbit polyclonal antibody.

    Labeling of IKK alpha + IKK beta with ab178870 is conducted at 1/50 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) secondary antibody (ab150077) at 1/200 dilution.
    Labeling of IKK alpha + IKK beta with the competitor antibody is conducted at 1/40 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor ® 488) at 1/200 dilution.

    Tubulin is detected with Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). The nuclear counter stain is DAPI (blue).
    The two negative controls were :
    -ve control 1 - ab178870 at 1/50 diltuion followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.
    -ve control 2 - Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution.

  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    All lanes : Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysates
    Lane 3 : HL-60 (Human promyelocytic leukemia cells) whole cell lysates

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 85, 87 kDa
    Observed band size: 75,87 kDa why is the actual band size different from the predicted?



    This western blot image is a comparison of ab178870 against a competitor's leading rabbit polyclonal antibody.

    Blocking/dilution buffer: 5% NFDM/TBST.

    ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.

  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    All lanes : Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200

    Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysates
    Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysates

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 85, 87 kDa
    Observed band size: 75,87 kDa why is the actual band size different from the predicted?



    This western blot image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.

    Blocking/dilution buffer: 5% NFDM/TBST.

    ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.

  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200 dilution + Human fetal kidney lysates

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 85, 87 kDa
    Observed band size: 87 kDa why is the actual band size different from the predicted?



    This western blot image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.

    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Western blot - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    All lanes : Anti-IKK alpha + IKK beta [EPR16628] antibody (ab178870) at 1/1000 dilution, and a competitor's Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/200 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse kidney lysates
    Lane 3 : Mouse spleen lysates
    Lane 4 : Rat kidney lysates
    Lane 5 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 85, 87 kDa
    Observed band size: 75,87 kDa why is the actual band size different from the predicted?



    This western blot image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.

    Blocking/dilution buffer: 5% NFDM/TBST.

    ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.

  • Immunoprecipitation - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Immunoprecipitation - Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)

    This immunoprecipation image is a comparison between ab178870 and a competitor's leading rabbit polyclonal antibody.

    Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using ab178870 at 1/40 dilution. Western blot detection was performed using ab178870 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and diluting buffer was 5% NFDM/TBST.

    Immunoprecipitation of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extracts using competitor’s Anti-IKK alpha + IKK beta rabbit polyclonal antibody at 1/12 dilution. Western blot detection was performed using competitor antibody at 1/100 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/100 dilution. The blocking and diluting buffer was 5% NFDM/TBST. 

    ab178870 could recognize 3 isoforms of IKK beta with the MWs of 87kDa, 86kDa and 80kDa, respectively. It could also recognize IKK alpha.

     

  • Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)
    Anti-IKK alpha + IKK beta antibody [EPR16628] (ab178870)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (40)

Publishing research using ab178870? Please let us know so that we can cite the reference in this datasheet.

ab178870 has been referenced in 40 publications.

  • Guo S  et al. Astaxanthin protects against early acute kidney injury in severely burned rats by inactivating the TLR4/MyD88/NF-?B axis and upregulating heme oxygenase-1. Sci Rep 11:6679 (2021). PubMed: 33758309
  • Chen G  et al. bFGF alleviates diabetes-associated endothelial impairment by downregulating inflammation via S-nitrosylation pathway. Redox Biol 41:101904 (2021). PubMed: 33706169
  • Yang J  et al. The inhibitory effect of Gualou Guizhi Decoction on post-ischemic neuroinflammation via miR-155 in MCAO rats. Ann Palliat Med 10:1370-1379 (2021). PubMed: 33081480
  • Sun J  et al. Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice. Pharm Biol 59:672-682 (2021). PubMed: 34078224
  • Zhang Q  et al. Humanized anti-TLR4 monoclonal antibody ameliorates lipopolysaccharide-related acute kidney injury by inhibiting TLR4/NF-?B signaling. Mol Med Rep 24:N/A (2021). PubMed: 34184086
View all Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Immunocytochemistry/ Immunofluorescence abreview for Anti-IKK alpha + IKK beta antibody [EPR16628]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.01% TritonX-100
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 06 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-IKK alpha + IKK beta antibody [EPR16628]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (H9c2 rat cardiomyocytes)
Permeabilization
Yes - 0.01% TritonX-100
Specification
H9c2 rat cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 23 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-IKK alpha + IKK beta antibody [EPR16628]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cardiomyocytes)
Permeabilization
Yes - 0.01% TritonX-100
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 18 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-IKK alpha + IKK beta [EPR16628] antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A375 (melanoma cell line))
Permeabilization
Yes - Triton 100x 0,5%
Specification
A375 (melanoma cell line)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Cristina Penas Lago

Verified customer

Submitted Dec 11 2017

Western blot abreview for Anti-IKK alpha + IKK beta [EPR16628] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (mesenchymal stem cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
mesenchymal stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kate Hawkins

Verified customer

Submitted Mar 22 2017

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