Recombinant
RabMAb

Recombinant Anti-IKK alpha + IKK beta antibody [EPR16628] (Alexa Fluor® 647) (ab200529)

Overview

  • Product name

    Anti-IKK alpha + IKK beta antibody [EPR16628] (Alexa Fluor® 647)
    See all IKK alpha + IKK beta primary antibodies
  • Description

    Rabbit monoclonal [EPR16628] to IKK alpha + IKK beta (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human IKK alpha + IKK beta aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: O14920

  • Positive control

    • ICC/IF: HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab200529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.

Target

  • Relevance

    IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta). IKK alpha and IKK beta interact with each other and both are essential for NFkB activation.
  • Cellular localization

    Cytoplasmic and Nuclear; Membrane raft. Note: IKK beta is colocalized with DPP4 in membrane rafts.
  • Database links

  • Alternative names

    • CHUK antibody
    • Conserved helix loop helix ubiquitous kinase antibody
    • Conserved helix-loop-helix ubiquitous kinase antibody
    • I kappa B kinase 1 antibody
    • I kappa B kinase 2 antibody
    • I Kappa B kinase alpha antibody
    • I Kappa B kinase beta antibody
    • IkB kinase alpha subunit antibody
    • IkBKA antibody
    • IkBKB antibody
    • IKK a kinase antibody
    • IKK alpha antibody
    • IKK beta antibody
    • IKK-alpha antibody
    • IKK-beta antibody
    • IKK1 antibody
    • IKK2 antibody
    • IKKA antibody
    • IKKB antibody
    • IMD15 antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
    • NFKBIKA antibody
    • NFKBIKB antibody
    • Nuclear factor NF kappa B inhibitor kinase beta antibody
    • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
    • Nuclear factor NFkappaB inhibitor kinase alpha antibody
    • TCF 16 antibody
    • TCF-16 antibody
    • TCF16 antibody
    • Transcription factor 16 antibody
    see all

Images

  • ab200529 staining IKK alpha+ IKK beta in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200529 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab200529 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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