Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody (ab194528)
Key features and details
- Rabbit polyclonal to IKK alpha + IKK beta (phospho S176 + S177)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody
See all IKK alpha + IKK beta primary antibodies -
Description
Rabbit polyclonal to IKK alpha + IKK beta (phospho S176 + S177) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human IKK alpha + IKK beta (phospho S176 + S177) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of serine 176/177 (Q-G-S(p)-L-C) derived from Human IKK alpha + IKK beta. SwissProt: O15111/O14920.
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Positive control
- WB: NIH 3T3 cell extracts, treated with TNF.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS (without Mg2+and Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab194528 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 85 kDa.
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| Notes |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 85 kDa. |
Target
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Relevance
IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta). IKK alpha and IKK beta interact with each other and both are essential for NFkB activation. -
Cellular localization
Cytoplasmic and Nuclear; Membrane raft. Note: IKK beta is colocalized with DPP4 in membrane rafts. -
Database links
- Entrez Gene: 1147 Human
- Entrez Gene: 3551 Human
- Entrez Gene: 12675 Mouse
- Entrez Gene: 16150 Mouse
- Omim: 600664 Human
- Omim: 603258 Human
- SwissProt: O14920 Human
- SwissProt: O15111 Human
see all -
Alternative names
- CHUK antibody
- Conserved helix loop helix ubiquitous kinase antibody
- Conserved helix-loop-helix ubiquitous kinase antibody
see all
Images
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Western blot - Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody (ab194528)All lanes : Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody (ab194528) at 1/500 dilution
Lane 1 : NIH 3T3 cell extract, treated with TNF
Lane 2 : NIH 3T3 cell extract, treated with TNF with antigen-specific peptide
Predicted band size: 85 kDa
Datasheets and documents
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Datasheet download
References (16)
ab194528 has been referenced in 16 publications.
- Teng S et al. The Protection of Crocin Against Ulcerative Colitis and Colorectal Cancer via Suppression of NF-?B-Mediated Inflammation. Front Pharmacol 12:639458 (2021). PubMed: 33841156
- Wang Z et al. Hypoxia-induced PLOD1 overexpression contributes to the malignant phenotype of glioblastoma via NF-?B signaling. Oncogene N/A:N/A (2021). PubMed: 33420370
- Sun J et al. Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice. Pharm Biol 59:672-682 (2021). PubMed: 34078224
- Liu C et al. Serum-derived exosomes from house dust mite-sensitized guinea pigs contribute to inflammation in BEAS-2B cells via the TLR4-NF-?B pathway. Mol Med Rep 24:N/A (2021). PubMed: 34458929
- Dong X et al. Glutathione S-transferases P1-mediated interleukin-6 in tumor-associated macrophages augments drug-resistance in MCF-7 breast cancer. Biochem Pharmacol 182:114289 (2020). PubMed: 33080187
