Overview

  • Product name
    Anti-IKK alpha antibody [ABM10G9]
    See all IKK alpha primary antibodies
  • Description
    Mouse monoclonal [ABM10G9] to IKK alpha
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Positive control
    • WB: HeLa, MCF7, U-87 MG, PC-3 and HEK-293 cell lysates; mouse small intestine lysate. Flow Cyt: Jurkat, HEK-293 and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 85 kDa.
Flow Cyt Use 0.5-1µg for 106 cells.

Target

  • Function
    Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
  • Tissue specificity
    Widely expressed.
  • Involvement in disease
    Defects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
    Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • chuk antibody
    • CHUK1 antibody
    • Conserved Helix Loop Helix Ubiquitous Kinase antibody
    • Conserved helix loop ubiquitous kinase antibody
    • Conserved helix-loop-helix ubiquitous kinase antibody
    • I Kappa B Kinase 1 antibody
    • I Kappa B Kinase Alpha antibody
    • I-kappa-B kinase 1 antibody
    • I-kappa-B kinase alpha antibody
    • IkappaB kinase antibody
    • IkB kinase alpha subunit antibody
    • IkBKA antibody
    • IKK 1 antibody
    • IKK A antibody
    • IKK a kinase antibody
    • IKK-A antibody
    • IKK-alpha antibody
    • IKK1 antibody
    • IKKA antibody
    • IKKA_HUMAN antibody
    • Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody
    • Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
    • NFKBIKA antibody
    • Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody
    • Nuclear factor NF kappa B inhibitor kinase alpha antibody
    • Nuclear factor NF-kappa-B inhibitor kinase alpha antibody
    • Nuclear factor NFkappaB inhibitor kinase alpha antibody
    • Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody
    • TCF-16 antibody
    • TCF16 antibody
    • Transcription factor 16 antibody
    see all

Images

  • All lanes : Anti-IKK alpha antibody [ABM10G9] (ab227852) at 2 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 4 : Mouse small intestine lysate
    Lane 5 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
    Lane 6 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Predicted band size: 85 kDa

  • Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling IKK alpha with ab227852 at 0.5 µg/106 cells (red) compared with an isotype control (green). Goat anti-mouse PE conjugate was used as secondary antibody.

  • Flow cytometric analysis of HEK-293 (human epithelial cell line from embryonic kidney) cell line labeling IKK alpha with ab227852 at 0.5 µg/106 cells (red) compared with an isotype control (green). Goat anti-mouse PE conjugate was used as secondary antibody.

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling IKK alpha with ab227852 at 0.5 µg/106 cells (red) compared with an isotype control (green). Goat anti-mouse PE conjugate was used as secondary antibody.

References

ab227852 has not yet been referenced specifically in any publications.

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