Overview

  • Product name

    Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody
    See all IKK alpha + IKK beta primary antibodies
  • Description

    Rabbit polyclonal to IKK alpha + IKK beta (phospho S176 + S177)
  • Host species

    Rabbit
  • Specificity

    ab194528 detects endogenous level of IKK alpha + IKK beta only when phosphorylated at serine 176/177.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human IKK alpha + IKK beta (phospho S176 + S177) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of serine 176/177 (Q-G-S(p)-L-C) derived from Human IKK alpha + IKK beta. SwissProt: O15111/O14920.

  • Positive control

    • NIH 3T3 cell extracts, treated with TNF.

Properties

Applications

Our Abpromise guarantee covers the use of ab194528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 85 kDa.

Target

  • Relevance

    IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta). IKK alpha and IKK beta interact with each other and both are essential for NFkB activation.
  • Cellular localization

    Cytoplasmic and Nuclear; Membrane raft. Note: IKK beta is colocalized with DPP4 in membrane rafts.
  • Database links

  • Alternative names

    • CHUK antibody
    • Conserved helix loop helix ubiquitous kinase antibody
    • Conserved helix-loop-helix ubiquitous kinase antibody
    • I kappa B kinase 1 antibody
    • I kappa B kinase 2 antibody
    • I Kappa B kinase alpha antibody
    • I Kappa B kinase beta antibody
    • IkB kinase alpha subunit antibody
    • IkBKA antibody
    • IkBKB antibody
    • IKK a kinase antibody
    • IKK alpha antibody
    • IKK beta antibody
    • IKK-alpha antibody
    • IKK-beta antibody
    • IKK1 antibody
    • IKK2 antibody
    • IKKA antibody
    • IKKB antibody
    • IMD15 antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
    • NFKBIKA antibody
    • NFKBIKB antibody
    • Nuclear factor NF kappa B inhibitor kinase beta antibody
    • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
    • Nuclear factor NFkappaB inhibitor kinase alpha antibody
    • TCF 16 antibody
    • TCF-16 antibody
    • TCF16 antibody
    • Transcription factor 16 antibody
    see all

Images

  • All lanes : Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody (ab194528) at 1/500 dilution

    Lane 1 : NIH 3T3 cell extract, treated with TNF
    Lane 2 : NIH 3T3 cell extract, treated with TNF with antigen-specific peptide

    Predicted band size: 85 kDa

References

This product has been referenced in:

  • Wang F  et al. Oscillating flow promotes inflammation through the TLR2-TAK1-IKK2 signalling pathway in human umbilical vein endothelial cell (HUVECs). Life Sci 224:212-221 (2019). Read more (PubMed: 30914314) »
  • Pichavaram P  et al. Cholesterol crystals promote endothelial cell and monocyte interactions via H2O2-mediated PP2A inhibition, NF?B activation and ICAM1 and VCAM1 expression. Redox Biol 24:101180 (2019). Read more (PubMed: 31022672) »
See all 6 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (mesenchymal stem cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
mesenchymal stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Kate Hawkins

Verified customer

Submitted Mar 22 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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