Key features and details
- Rabbit polyclonal to IKK alpha (phospho S176 + S180)
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IKK alpha (phospho S176 + S180) antibody
See all IKK alpha primary antibodies
DescriptionRabbit polyclonal to IKK alpha (phospho S176 + S180)
SpecificityDue to sequence homology between the isoforms, this antibody may cross-react with IKKbeta that contains phosphorylated serine 176 and 180. However, we have no direct experimental evidence for this in our records.
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phosphopeptide derived from the region of human IKKalpha that contains serine 176 and 180.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
PBS is Ca2+ and Mg2+ free
Concentration information loading...
PurityImmunogen affinity purified
FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
Tissue specificityWidely expressed.
Involvement in diseaseDefects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
Cellular localizationCytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
- Information by UniProt
- chuk antibody
- CHUK1 antibody
- Conserved Helix Loop Helix Ubiquitous Kinase antibody
ab17943 has been referenced in 8 publications.
- Zhang Y et al. Glycyrrhetinic Acid Improves Insulin-Response Pathway by Regulating the Balance between the Ras/MAPK and PI3K/Akt Pathways. Nutrients 11:N/A (2019). PubMed: 30871060
- Zhang Y et al. HZ08 suppresses RelB-activated MnSOD expression and enhances Radiosensitivity of prostate Cancer cells. J Exp Clin Cancer Res 37:174 (2018). PubMed: 30053873
- Noort AR et al. NF-?B-inducing kinase is a key regulator of inflammation-induced and tumour-associated angiogenesis. J Pathol 234:375-85 (2014). PubMed: 25043127
- Chen K et al. Germline mutations in NFKB2 implicate the noncanonical NF-?B pathway in the pathogenesis of common variable immunodeficiency. Am J Hum Genet 93:812-24 (2013). PubMed: 24140114
- Yakubov B et al. Extracellular tissue transglutaminase activates noncanonical NF-?B signaling and promotes metastasis in ovarian cancer. Neoplasia 15:609-19 (2013). WB . PubMed: 23730209
- Chung S et al. NF-?B Inducing Kinase, NIK Mediates Cigarette Smoke/TNFa-Induced Histone Acetylation and Inflammation through Differential Activation of IKKs. PLoS One 6:e23488 (2011). WB, ICC/IF ; Human . PubMed: 21887257
- Khan KA et al. Bcl-3-regulated transcription from major immediate-early promoter of human cytomegalovirus in monocyte-derived macrophages. J Immunol 182:7784-94 (2009). WB ; Human . PubMed: 19494302
- Yang SR et al. IKK alpha causes chromatin modification on pro-inflammatory genes by cigarette smoke in mouse lung. Am J Respir Cell Mol Biol 38:689-98 (2008). PubMed: 18239189