Overview

  • Product name
    Anti-IKK alpha (phospho S176 + S180) antibody
    See all IKK alpha primary antibodies
  • Description
    Rabbit polyclonal to IKK alpha (phospho S176 + S180)
  • Host species
    Rabbit
  • Specificity
    Due to sequence homology between the isoforms, this antibody may cross-react with IKKbeta that contains phosphorylated serine 176 and 180. However, we have no direct experimental evidence for this in our records.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from the region of human IKKalpha that contains serine 176 and 180.

Properties

Applications

Our Abpromise guarantee covers the use of ab17943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21887257
WB Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 85 kDa. When looking at the phosphorylation of endogenous proteins, we recommend that IKKalpha first be immunoprecipitated.

Target

  • Function
    Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
  • Tissue specificity
    Widely expressed.
  • Involvement in disease
    Defects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
    Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • chuk antibody
    • CHUK1 antibody
    • Conserved Helix Loop Helix Ubiquitous Kinase antibody
    • Conserved helix loop ubiquitous kinase antibody
    • Conserved helix-loop-helix ubiquitous kinase antibody
    • I Kappa B Kinase 1 antibody
    • I Kappa B Kinase Alpha antibody
    • I-kappa-B kinase 1 antibody
    • I-kappa-B kinase alpha antibody
    • IkappaB kinase antibody
    • IkB kinase alpha subunit antibody
    • IkBKA antibody
    • IKK 1 antibody
    • IKK A antibody
    • IKK a kinase antibody
    • IKK-A antibody
    • IKK-alpha antibody
    • IKK1 antibody
    • IKKA antibody
    • IKKA_HUMAN antibody
    • Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody
    • Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
    • NFKBIKA antibody
    • Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody
    • Nuclear factor NF kappa B inhibitor kinase alpha antibody
    • Nuclear factor NF-kappa-B inhibitor kinase alpha antibody
    • Nuclear factor NFkappaB inhibitor kinase alpha antibody
    • Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody
    • TCF-16 antibody
    • TCF16 antibody
    • Transcription factor 16 antibody
    see all

References

This product has been referenced in:
  • Zhang Y  et al. HZ08 suppresses RelB-activated MnSOD expression and enhances Radiosensitivity of prostate Cancer cells. J Exp Clin Cancer Res 37:174 (2018). Read more (PubMed: 30053873) »
  • Noort AR  et al. NF-?B-inducing kinase is a key regulator of inflammation-induced and tumour-associated angiogenesis. J Pathol 234:375-85 (2014). Read more (PubMed: 25043127) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry.

I can confirm that as ab105923IKK alpha antibodyhas not specifically been raised against a phosphorylated residue of the protein, it is therefore likely to detect both phosphorylated and non phosphorylated forms of the protein.

We do have the following antibodies that detect some of the phosphorylated residues specifically. I can recommend to review the datasheets for further details and to select one tested in the species you wil be using.

ab38515 Anti-IKK alpha (phospho T23) antibody
Rabbit polyclonal
Applications: ELISA, ICC/IF, IHC-Fr, IHC-P, WB
Reacts with: Human, Mouse, Rat
https://www.abcam.com/index.html?datasheet=38515 (or use the following: https://www.abcam.com/index.html?datasheet=38515).

ab17943 Anti-IKK alpha (phospho S176 + S180) antibody
Rabbit polyclonal
Applications: ICC/IF, WB
Reacts with: Human
https://www.abcam.com/index.html?datasheet=17943 (or use the following: https://www.abcam.com/index.html?datasheet=17943).

I am sorry that as far as we are aware, these have not been tested and therefore are not guaranteed in flow cytometry.However, if you would like to test these antibodies in flow cystometry, please do not hesitate to contact me again prior to the purchase by replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (Intestine (colon, jejunum and Ileum))
Loading amount
50 µg
Specification
Intestine (colon, jejunum and Ileum)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Ms. Ananda Rodrigues

Verified customer

Submitted Aug 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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