I made a test running different amount of protein (10, 20, 30, 40 and 50 µg of protein) in the same gel. I had a lot non-specific binding and I tried different dilutions with the secondary antibody and did not work better.
It was used for the WB: PVDF menbrane, the Complete Protease Inhibitor Cocktail from Roche (cat no: 04693/24001), Lysis buffer from thermo Scientfic (Tissue protein extractio, cat no: 78510), ECL from Pierce (cat n°: PI32106) and Film Hyblot from Denville Scientific (cat no: E3012).
This customer received a testing discount to try this antibody in pig, an untested species. The preliminary data suggest that this antibody may not be suitable for detection of the pig protein.
Ms. Ananda Rodrigues
Submitted Aug 02 2010
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