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Cell Biology Apoptosis Intracellular NFkB IKK
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Anti-IKK alpha (phospho S176) antibody (ab138426)

  • Datasheet
  • SDS
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Western blot - Anti-IKK alpha (phospho S176) antibody (ab138426)

    Key features and details

    • Rabbit polyclonal to IKK alpha (phospho S176)
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

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    Overview

    • Product name

      Anti-IKK alpha (phospho S176) antibody
      See all IKK alpha primary antibodies
    • Description

      Rabbit polyclonal to IKK alpha (phospho S176)
    • Host species

      Rabbit
    • Specificity

      ab138426 detects endogenous levels of IKK alpha only when phosphorylated at S176.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Rat
    • Immunogen

      Synthetic phosphopeptide against an internal region around S176 of Human IKK alpha (UniProt ID: O15111).

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20ºC.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

      PBS is without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab138426 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • NFkB
      • IKK
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • NFkB Pathway
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Inflammatory mediators
      • Immunology
      • Innate Immunity
      • TLR Signaling
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • NFkB
      • IKK

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • NIH/3T3 cytoplasmic extract lysate (ab14873)
      • NIH/3T3 nuclear extract lysate (ab14874)
      • NIH/3T3 whole cell lysate (ab7179)
    • Recombinant Protein

      • Recombinant human IKK alpha protein (Active) (ab102103)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab138426 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    1/500 - 1/1000. Predicted molecular weight: 84 kDa.
    Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 84 kDa.

    Target

    • Function

      Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
    • Tissue specificity

      Widely expressed.
    • Involvement in disease

      Defects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
      Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
    • Cellular localization

      Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
    • Target information above from: UniProt accession O15111 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 309361 Rat
      • SwissProt: Q60680 Mouse
      • Unigene: 3996 Mouse
      • Alternative names

        • chuk antibody
        • CHUK1 antibody
        • Conserved Helix Loop Helix Ubiquitous Kinase antibody
        • Conserved helix loop ubiquitous kinase antibody
        • Conserved helix-loop-helix ubiquitous kinase antibody
        • I Kappa B Kinase 1 antibody
        • I Kappa B Kinase Alpha antibody
        • I-kappa-B kinase 1 antibody
        • I-kappa-B kinase alpha antibody
        • IkappaB kinase antibody
        • IkB kinase alpha subunit antibody
        • IkBKA antibody
        • IKK 1 antibody
        • IKK A antibody
        • IKK a kinase antibody
        • IKK-A antibody
        • IKK-alpha antibody
        • IKK1 antibody
        • IKKA antibody
        • IKKA_HUMAN antibody
        • Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody
        • Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody
        • Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
        • NFKBIKA antibody
        • Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody
        • Nuclear factor NF kappa B inhibitor kinase alpha antibody
        • Nuclear factor NF-kappa-B inhibitor kinase alpha antibody
        • Nuclear factor NFkappaB inhibitor kinase alpha antibody
        • Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody
        • TCF-16 antibody
        • TCF16 antibody
        • Transcription factor 16 antibody
        see all

      Images

      • Western blot - Anti-IKK alpha (phospho S176) antibody (ab138426)
        Western blot - Anti-IKK alpha (phospho S176) antibody (ab138426)
        Anti-IKK alpha (phospho S176) antibody (ab138426) at 1/500 dilution + extracts from NIH 3T3 cells treated with 20 ng/ml of TNF for 30 minutes

        Predicted band size: 84 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab138426? Please let us know so that we can cite the reference in this datasheet.

      ab138426 has been referenced in 3 publications.

      • Qu M  et al. Yi-Qi-Ping-Chuan-Fang Reduces TSLP Elevation Caused by LPS + Poly(I:C) via Inhibiting TLR4/MYD88/NF-?B Signaling Pathway. Evid Based Complement Alternat Med 2017:3209407 (2017). PubMed: 29250125
      • Wang C  et al. HTLV-1 Tax Functions as a Ubiquitin E3 Ligase for Direct IKK Activation via Synthesis of Mixed-Linkage Polyubiquitin Chains. PLoS Pathog 12:e1005584 (2016). PubMed: 27082114
      • Tao M  et al. Inflammatory stimuli promote growth and invasion of pancreatic cancer cells through NF-?B pathway dependent repression of PP2Ac. Cell Cycle 15:381-93 (2016). PubMed: 26761431

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Western blot abreview for Anti-IKK alpha (phospho S176) antibody

      Good
      Abreviews
      Abreviews
      Application
      Western blot
      Loading amount
      30 µg
      Gel Running Conditions
      Reduced Denaturing
      Sample
      Human Cell lysate - whole cell (macrophage)
      Specification
      macrophage
      Treatment
      0.1 ug/ml LPS 15 min
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 12 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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