Anti-IKK alpha (phospho T23) antibody (ab38515)
Key features and details
- Rabbit polyclonal to IKK alpha (phospho T23)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IKK alpha (phospho T23) antibody
See all IKK alpha primary antibodies -
Description
Rabbit polyclonal to IKK alpha (phospho T23) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IKK alpha aa 1-100 (phospho T23).
Database link: O15111 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1147 Human
- Omim: 600664 Human
- SwissProt: O15111 Human
- Unigene: 198998 Human
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Alternative names
- chuk antibody
- CHUK1 antibody
- Conserved Helix Loop Helix Ubiquitous Kinase antibody
see all
Images
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Western blot - Anti-IKK alpha (phospho T23) antibody (ab38515)All lanes : Anti-IKK alpha (phospho T23) antibody (ab38515) at 1/500 dilution
Lane 1 : MDA-MB-435 cell extract.
Lane 2 : MDA-MB-435 cell extract + EGF ( The cells were treated with 200ng/ml EGF for 5 minutes).
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)
Predicted band size: 85 kDa
Observed band size: 85 kDa
Lanes can be loaded with 5-30µg of total protein. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK alpha (phospho T23) antibody (ab38515)ab38515 at a 1:50 dilution staining IKK alpha in Human colon carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.Left image : untreated.
Right image : treated with phosphopeptide.
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Immunocytochemistry/ Immunofluorescence - Anti-IKK alpha (phospho T23) antibody (ab38515)ICC/IF image of ab38515 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38515, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab38515 has been referenced in 33 publications.
- Shen H et al. PTOV1 promotes cisplatin-induced chemotherapy resistance by activating the nuclear factor kappa B pathway in ovarian cancer. Mol Ther Oncolytics 20:499-507 (2021). PubMed: 33738336
- Zhang WB et al. Protective Effects of Oroxylin A against Doxorubicin-Induced Cardiotoxicity via the Activation of Sirt1 in Mice. Oxid Med Cell Longev 2021:6610543 (2021). PubMed: 33542782
- Li Q et al. Carminic acid supplementation protects against fructose-induced kidney injury mainly through suppressing inflammation and oxidative stress via improving Nrf-2 signaling. Aging (Albany NY) 13:10326-10353 (2021). PubMed: 33819919
- Tian S et al. Curcumin inhibits the growth of liver cancer by impairing myeloid-derived suppressor cells in murine tumor tissues. Oncol Lett 21:286 (2021). PubMed: 33732362
- Chen X et al. Eupafolin alleviates cerebral ischemia/reperfusion injury in rats via blocking the TLR4/NF-?B signaling pathway. Mol Med Rep 22:5135-5144 (2020). PubMed: 33173992