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I have a query regarding your IKK-alpha antibody (code-ab105923,product name-Anti-IKK alpha antibody [3G12H9])...I want to use this antibody for flow cytometric analysis of phosphorylated IKK-alpha, but do your antibody detect phosphorylated IKK-alpha or only IKK-alpha? becuase I want to study the TLR signalling pathway and for this I want to look at activated (phosphorylated) IIKK alpha enzyme. So can you guide me regarding this...so that I can order this antibody accordingly?
Waiting for you reply..
Asked on Jan 24 2012
Thank you for your enquiry.
I can confirm that as ab105923IKK alpha antibodyhas not specifically been raised against a phosphorylated residue of the protein, it is therefore likely to detect both phosphorylated and non phosphorylated forms of the protein.
We do have the following antibodies that detect some of the phosphorylated residues specifically. I can recommend to review the datasheets for further details and to select one tested in the species you wil be using.
ab38515 Anti-IKK alpha (phospho T23) antibody
Applications: ELISA, ICC/IF, IHC-Fr, IHC-P, WB
Reacts with: Human, Mouse, Rat
https://www.abcam.com/index.html?datasheet=38515 (or use the following: https://www.abcam.com/index.html?datasheet=38515).
ab17943 Anti-IKK alpha (phospho S176 + S180) antibody
Applications: ICC/IF, WB
Reacts with: Human
https://www.abcam.com/index.html?datasheet=17943 (or use the following: https://www.abcam.com/index.html?datasheet=17943).
I am sorry that as far as we are aware, these have not been tested and therefore are not guaranteed in flow cytometry.However, if you would like to test these antibodies in flow cystometry, please do not hesitate to contact me again prior to the purchase by replying to this message as you may be eligible for our testing discount program.
Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.
To find out more about our Abreview system, please see the following link:
I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.
Answered on Jan 24 2012