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I used this antibody in WB of various human fibroblast cell lines. I detect a single band, but it is at 130kDa, rather than 85 - is this the correct band?
Asked on Jan 31 2012
Thank you for contacting us regarding ab38515.
I have received the following additional information from the lab:
This target containing leucine zipper associates with many proteins. It is posible that theband is dimerization. The leucine zipper is important to allow homo- and hetero-dimerization. Add DTT to lysis buffer as we find this helps.
I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.
Answered on Jan 31 2012