Question (34772) | Anti-IKK alpha (phospho T23) antibody (ab38515)

Go to datasheet (ab38515)

Question

I used this antibody in WB of various human fibroblast cell lines. I detect a single band, but it is at 130kDa, rather than 85 - is this the correct band?

Answer

Thank you for contacting us regarding ab38515.

I have received the following additional information from the lab:

This target containing leucine zipper associates with many proteins. It is posible that theband is dimerization. The leucine zipper is important to allow homo- and hetero-dimerization. Add DTT to lysis buffer as we find this helps.

I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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