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human skin tissue, IHC-P
mouse skin tissue, IHC-Fr
no signal at all
Ab from other company gave desired staining
same standard protocol for both Abs:
AR: citrate buffer
Ab: 1/25, 1/50, 1/75, 1/100 several incubation times
secondary: HRP or polymer
blocking: not sure
Asked on Apr 23 2012
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Answered on Apr 23 2012