Western blot abreview for Anti-IKK alpha (phospho T23) antibody

Poor
Abreviews
Application
Western blot
Sample
Guinea pig Tissue lysate - whole (myometrium pregnant and non-pregnant)
Loading amount
30 µg
Specification
myometrium pregnant and non-pregnant
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL prime
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 50, 70 kDa
Positive control
WISH cells LPS 20ug/ml, 30 ug/lane

Additional data

Additional Notes
samples on the gel:1) non-pregnant myometrium guinea pig, 30ug
2)pregnant myometrium guinea pig,30ug
3)non-pregnant myometrium human, 30ug
4)pregnant myometrium human, 30ug
5) Wish cells/LPS stimulated
Abcam response
Thank you so much for testing this antibody in guinea pig. We appreciate very much your efforts, but are sorry to hear that the results were not satisfactory. The customer received a discount on a future antibody purchase for testing this product in guinea pig. We would welcome additional feedback from other customers as well.

Abcam user community

Verified customer

Submitted Feb 07 2012

Sign up