Anti-IKK beta antibody [EPR6043] - BSA and Azide free KO Tested Recombinant (ab171364)

Product name

Anti-IKK beta antibody [EPR6043] - BSA and Azide free
See all IKK beta primary antibodies
Description

Rabbit monoclonal [EPR6043] to IKK beta - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
General notes

ab171364 is a PBS-buffered version of ab124957, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab124957 for information on protocols, dilutions, and image data.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Constituent: PBS
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

EPR6043
Research areas
- Cell Biology
- Apoptosis
- Intracellular
- NFkB
- IKK
- Cancer
- Cell Death
- Apoptosis
- Apoptosis Markers
- NFkB
- IKK

Alternative Versions
- Anti-IKK beta antibody [EPR6043] (ab124957)

Our Abpromise guarantee covers the use of ab171364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		Use at an assay dependent concentration. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa). Can be blocked with ab154148
ICC/IF		Use at an assay dependent concentration.
Flow Cyt		Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)

Application notes

Is unsuitable for IP.

Function

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
Tissue specificity

Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications

Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
Cellular localization

Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
Target information above from: UniProt accession O14920 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3551 Human
- Entrez Gene: 16150 Mouse
- Entrez Gene: 84351 Rat
- Omim: 603258 Human
- SwissProt: O14920 Human
- SwissProt: O88351 Mouse
- SwissProt: Q9QY78 Rat
- Unigene: 597664 Human
- Unigene: 277886 Mouse
- Unigene: 19222 Rat
see all
Alternative names
- I kappa B kinase 2 antibody
- I kappa B kinase beta antibody
- I-kappa-B kinase 2 antibody
- I-kappa-B-kinase beta antibody
- IkBKB antibody
- IKK beta antibody
- IKK-B antibody
- IKK-beta antibody
- IKK2 antibody
- IKKB antibody
- IKKB_HUMAN antibody
- IMD15 antibody
- Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
- Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
- NFKBIKB antibody
- Nuclear factor NF-kappa-B inhibitor kinase beta antibody
see all

Flow Cytometry - Anti-IKK beta antibody [EPR6043] - BSA and Azide free (ab171364)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IKK beta with unpurified ab124957 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124957).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] - BSA and Azide free (ab171364)

ab124957, at 1/100 dilution staining IKK beta in paraffin-embedded Human cervix carcinoma tissue, by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124957).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] - BSA and Azide free (ab171364)

ab124957, at 1/100 dilution staining IKK beta in paraffin-embedded Mouse kidney tissue, by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124957).
Immunocytochemistry/ Immunofluorescence - Anti-IKK beta antibody [EPR6043] - BSA and Azide free (ab171364)

ab124957, at 1/50 dilution, staining IKK beta in HeLa cells by Immunofluorescence.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124957).
Other - Anti-IKK beta antibody [EPR6043] - BSA and Azide free (ab171364)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124957).
Western blot - Anti-IKK beta antibody [EPR6043] - BSA and Azide free (ab171364)

This WB data was generated using the same anti-IKK beta antibody clone [EPR6043] in a different buffer formulation (cat# ab124957).

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IKK beta knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab124957 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124957 was shown to specifically react with IKK beta when IKK beta knockout samples were used. Wild-type and IKK beta knockout samples were subjected to SDS-PAGE. ab124957 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

Datasheet

ab171364 has not yet been referenced specifically in any publications.

Publishing research using ab171364? Please let us know so that we can cite the reference in this datasheet.

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Anti-IKK beta antibody [EPR6043] - BSA and Azide free (ab171364)

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