Overview

  • Product name

    Anti-IKK beta (phospho Y188) antibody
    See all IKK beta primary antibodies
  • Description

    Rabbit polyclonal to IKK beta (phospho Y188)
  • Host species

    Rabbit
  • Specificity

    ab194519 detects endogenous levels of IKK beta only when phosphorylated at tyrosine 188.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human IKK beta (phospho Y188) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    L-Q-Y(p)-L-A


    Database link: O14920

  • Positive control

    • COS7 cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab194519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 87 kDa.

Target

  • Function

    Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
  • Tissue specificity

    Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
    Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
    Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
  • Cellular localization

    Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • I kappa B kinase 2 antibody
    • I kappa B kinase beta antibody
    • I-kappa-B kinase 2 antibody
    • I-kappa-B-kinase beta antibody
    • IkBKB antibody
    • IKK beta antibody
    • IKK-B antibody
    • IKK-beta antibody
    • IKK2 antibody
    • IKKB antibody
    • IKKB_HUMAN antibody
    • IMD15 antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
    • NFKBIKB antibody
    • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
    see all

Images

  • All lanes : Anti-IKK beta (phospho Y188) antibody (ab194519) at 1/500 dilution

    Lane 1 : COS7 cell extract
    Lane 2 : COS7 cell extract with antigen-specific peptide

    Predicted band size: 87 kDa

References

This product has been referenced in:

  • Jiang L  et al. Overexpression of PIMREG promotes breast cancer aggressiveness via constitutive activation of NF-?B signaling. EBioMedicine 43:188-200 (2019). Read more (PubMed: 30979686) »
  • Jiang Y  et al. TIPE2 governs macrophage polarization via negative regulation of mTORC1. Mol Med Rep 17:952-960 (2018). Read more (PubMed: 29115630) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Ixodes scapularis Cell lysate - whole cell (IDE12)
Gel Running Conditions
Reduced Denaturing
Loading amount
80 µg
Specification
IDE12
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 20 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Macrophage Cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Macrophage Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 17 2018

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