Key features and details
- Rabbit polyclonal to IKK beta (phospho Y188)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IKK beta (phospho Y188) antibody
See all IKK beta primary antibodies
DescriptionRabbit polyclonal to IKK beta (phospho Y188)
Specificityab53694 detects endogenous levels of IKK beta only when phosphorylated at tyrosine 188.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Synthetic phosphopeptide derived from human IKK beta around the phosphorylation site of tyrosine 188 (L-Q-YP-L-A).
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab53694 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
Tissue specificityHighly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
modificationsUpon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
Cellular localizationCytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
- I kappa B kinase 2 antibody
- I kappa B kinase beta antibody
- I-kappa-B kinase 2 antibody
ab53694 has been referenced in 3 publications.
- Wang Z et al. ERa upregulates the expression of long non-coding RNA LINC00472 which suppresses the phosphorylation of NF-?B in breast cancer. Breast Cancer Res Treat 175:353-368 (2019). PubMed: 30830488
- Wang X et al. Aspirin ameliorates cerebral infarction through regulation of TLR4/NF-?B-mediated endoplasmic reticulum stress in mouse model. Mol Med Rep 17:479-487 (2018). PubMed: 29115440
- Purkayastha S et al. Uncoupling the mechanisms of obesity and hypertension by targeting hypothalamic IKK-ß and NF-?B. Nat Med 17:883-7 (2011). IHC ; Mouse . PubMed: 21642978