Overview

  • Product name
    Anti-IKK beta (phospho Y199) antibody
    See all IKK beta primary antibodies
  • Description
    Rabbit polyclonal to IKK beta (phospho Y199)
  • Host species
    Rabbit
  • Specificity
    Detects endogenous levels of IKK beta only when phosphorylated at tyrosine 199.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from around the phosphorylation site of tyrosine 199 (QKYPTV) of human IKK beta

  • Positive control
    • Human breast carcinoma tissue HeLa cells treated with TNF-alpha and calyculin

Properties

Applications

Our Abpromise guarantee covers the use of ab59195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.

Target

  • Function
    Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
  • Tissue specificity
    Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
    Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
    Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
  • Cellular localization
    Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • I kappa B kinase 2 antibody
    • I kappa B kinase beta antibody
    • I-kappa-B kinase 2 antibody
    • I-kappa-B-kinase beta antibody
    • IkBKB antibody
    • IKK beta antibody
    • IKK-B antibody
    • IKK-beta antibody
    • IKK2 antibody
    • IKKB antibody
    • IKKB_HUMAN antibody
    • IMD15 antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
    • NFKBIKB antibody
    • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59195 at 1/50 dilution. Left image: untreated. Right image: sample treated with immunising peptide.
  • All lanes : Anti-IKK beta (phospho Y199) antibody (ab59195) at 1/500 dilution

    Lane 1 : HeLa cell extracts treated with TNF alpha (20ng/ml) and calyculin (50nM, 5 mins)
    Lane 2 : HeLa cell extracts treated with TNF alpha (20ng/ml) and calyculin (50nM, 5 mins) with phosphopeptide

    Predicted band size: 87 kDa
    Observed band size: 87 kDa

References

This product has been referenced in:
  • Jiang P  et al. Astragaloside IV Prevents Obesity-Associated Hypertension by Improving Pro-Inflammatory Reaction and Leptin Resistance. Mol Cells 41:244-255 (2018). Read more (PubMed: 29562733) »
  • Yang T  et al. lncRNA-NKILA/NF-?B feedback loop modulates laryngeal cancer cell proliferation, invasion, and radioresistance. Cancer Med 7:2048-2063 (2018). WB ; Human . Read more (PubMed: 29573243) »

See all 12 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Treatment
0.1 ug/ml LPS 15 min
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (prostate adenocarcinoma)
Specification
prostate adenocarcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA (pH 9)
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Lewis MacKenzie

Verified customer

Submitted Feb 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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