• Product name

  • Description

    Rabbit polyclonal to IKKi/IKKe
  • Host species

  • Specificity

    Recognises IKK epsilon and does not cross react with IKK alpha, beta or gamma.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Peptide (


    ) corresponding to aminoacids 701 to 716 of human IKKe/IKK-i (1,2).

  • Positive control

    • Whole cell lysate from Jurkat cells
  • General notes

    Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. IkB is phosphorylated by IkB kinase(IKK) complex that contains IKKa, IKKb, and IKKg. A novel molecule in the IKK complex was recently identified and designated IKKe/IKK-i. IKKe is required for the activation of NF-kB by PMA and T cell receptors but not by TNFa and IL-1. IKKe/IKK-i message is expressed in a variety of tissues and is inducible by TNFa, IL-1, and LPS.



Our Abpromise guarantee covers the use of ab7891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
IP Use a concentration of 3 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 80 kDa.


  • Function

    Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
  • Tissue specificity

    Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational

    Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.
  • Information by UniProt
  • Database links

  • Alternative names

    • I kappa B kinase epsilon antibody
    • I-kappa-B kinase epsilon antibody
    • IkBKE antibody
    • IKK related kinase epsilon antibody
    • IKK-E antibody
    • IKK-epsilon antibody
    • IKK-i antibody
    • IKKE antibody
    • IKKE_HUMAN antibody
    • IKKepsilon antibody
    • IKKI antibody
    • Inducible I kappa B kinase antibody
    • Inducible I kappa-B kinase antibody
    • Inducible IkappaB kinase antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody
    • Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody
    • KIAA0151 antibody
    • MGC125294 antibody
    • MGC125295 antibody
    • MGC125297 antibody
    see all


  • Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml + Jurkat whole cell lysate

    Predicted band size: 80 kDa
    Observed band size: 80 kDa

  • ab7891 at 10µg/ml staining IKK epsilon in human pancreatic tissue by IHC

  • ICC/IF image of ab7891 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7891, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Yuan Y  et al. Long non-coding RNA cytoskeleton regulator RNA (CYTOR) modulates pathological cardiac hypertrophy through miR-155-mediated IKKi signaling. Biochim Biophys Acta Mol Basis Dis 1865:1421-1427 (2019). Read more (PubMed: 30794866) »
  • Xia H  et al. An evolutionary NS1 mutation enhances Zika virus evasion of host interferon induction. Nat Commun 9:414 (2018). Read more (PubMed: 29379028) »
See all 10 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (MCF7)
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 12 2009


This antibody has not yet been tested with mouse ikk epsilon.

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