Key features and details
- Mouse monoclonal [ABM13C7] to IKKi/IKKe - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
Product nameAnti-IKKi/IKKe antibody [ABM13C7] - N-terminal
See all IKKi/IKKe primary antibodies
DescriptionMouse monoclonal [ABM13C7] to IKKi/IKKe - N-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human IKKi/IKKe aa 2-203 (N terminal).
QSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVKVFNTTSYLRPREV QVREFEVLRKLNHQNIVKLFAVEETGGSRQKVLVMEYCSSGSLLSVLESP ENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQS IYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVT VD
Database link: Q14164
- Jurkat, HepG2 and mouse NIH 3T3 cell lysates; Human thyroid cancer tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab210927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 80 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionPhosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
Tissue specificityHighly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.
Cellular localizationCytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.
- Information by UniProt
- I kappa B kinase epsilon antibody
- I-kappa-B kinase epsilon antibody
- IkBKE antibody
All lanes : Anti-IKKi/IKKe antibody [ABM13C7] - N-terminal (ab210927) at 2 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : mouse NIH 3T3 cell lysate
Lane 3 : HepG2 cell lysate
Predicted band size: 80 kDa
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue, labeling IKKi/IKKe with ab210927 at 10 μg/mL.
ab210927 has been referenced in 1 publication.
- Wang P et al. Potential role of the IL17RC gene in the thoracic ossification of the posterior longitudinal ligament. Int J Mol Med 43:2005-2014 (2019). PubMed: 30864693