Product nameAnti-IKKi/IKKe antibody [EPR4904]
See all IKKi/IKKe primary antibodies
DescriptionRabbit monoclonal [EPR4904] to IKKi/IKKe
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human IKKi/IKKe aa 1-100. The exact sequence is proprietary.
- HeLa, Raji, MCF7 and Jurkat cell lysates; Human tonsil tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.52 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionPhosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
Tissue specificityHighly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.
Cellular localizationCytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.
- Information by UniProt
- I kappa B kinase epsilon antibody
- I-kappa-B kinase epsilon antibody
- IkBKE antibody
All lanes : Anti-IKKi/IKKe antibody [EPR4904] (ab124766) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 80 kDa
ab124766, at 1/100 dilution, staining IKKi/IKKe in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Yang W et al. Prognostic significance of preoperative IKBKE expression in esophageal squamous cell carcinoma. Onco Targets Ther 11:1305-1314 (2018). IHC-P . Read more (PubMed: 29563809) »
- Harquail J et al. Pax-5 Inhibits NF-?B Activity in Breast Cancer Cells Through IKKe and miRNA-155 Effectors. J Mammary Gland Biol Neoplasia 23:177-187 (2018). Read more (PubMed: 30032344) »