RecombinantRabMAb

Recombinant Anti-IKKi/IKKe antibody [EPR4904] (ab124766)

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Western blot - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)
  • OI-RD Scanning - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)
  • Anti-IKKi/IKKe antibody [EPR4904] (ab124766)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4904] to IKKi/IKKe
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-IKKi/IKKe antibody [EPR4904]
    See all IKKi/IKKe primary antibodies

  • Description

    Rabbit monoclonal [EPR4904] to IKKi/IKKe

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human IKKi/IKKe aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa, Raji, MCF7 and Jurkat whole cell lysate (ab7899); Human tonsil tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min).
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min).
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.

    • Tissue specificity

      Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.

    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Autophosphorylated.
      Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.

    • Cellular localization

      Cytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.
    • Information by UniProt

    • Database links

      • Alternative names

        • I kappa B kinase epsilon antibody
        • I-kappa-B kinase epsilon antibody
        • IkBKE antibody
        • IKK related kinase epsilon antibody
        • IKK-E antibody
        • IKK-epsilon antibody
        • IKK-i antibody
        • IKKE antibody
        • IKKE_HUMAN antibody
        • IKKepsilon antibody
        • IKKI antibody
        • Inducible I kappa B kinase antibody
        • Inducible I kappa-B kinase antibody
        • Inducible IkappaB kinase antibody
        • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody
        • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody
        • Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody
        • KIAA0151 antibody
        • MGC125294 antibody
        • MGC125295 antibody
        • MGC125297 antibody
        see all

      Images

      • Western blot - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)
        Western blot - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)
        All lanes : Anti-IKKi/IKKe antibody [EPR4904] (ab124766) at 1/10000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : Raji cell lysate
        Lane 3 : MCF7 cell lysate
        Lane 4 : Jurkat cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Predicted band size: 80 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)

        ab124766, at 1/100 dilution, staining IKKi/IKKe in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • OI-RD Scanning - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)
        OI-RD Scanning - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-IKKi/IKKe antibody [EPR4904] (ab124766)
        Anti-IKKi/IKKe antibody [EPR4904] (ab124766)

      References (3)

      Publishing research using ab124766? Please let us know so that we can cite the reference in this datasheet.

      ab124766 has been referenced in 3 publications.

      • Hudon Thibeault AA  et al. Serotonin and serotonin reuptake inhibitors alter placental aromatase. J Steroid Biochem Mol Biol 195:105470 (2019). PubMed: 31509772
      • Yang W  et al. Prognostic significance of preoperative IKBKE expression in esophageal squamous cell carcinoma. Onco Targets Ther 11:1305-1314 (2018). IHC-P . PubMed: 29563809
      • Harquail J  et al. Pax-5 Inhibits NF-?B Activity in Breast Cancer Cells Through IKKe and miRNA-155 Effectors. J Mammary Gland Biol Neoplasia 23:177-187 (2018). PubMed: 30032344

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