Recombinant Anti-IKKi/IKKe antibody [EPR4904] (ab124766)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-IKKi/IKKe antibody [EPR4904]
See all IKKi/IKKe primary antibodies -
Description
Rabbit monoclonal [EPR4904] to IKKi/IKKe -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human IKKi/IKKe aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa, Raji, MCF7 and Jurkat whole cell lysate (ab7899); Human tonsil tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.52 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4904 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min). | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/100 - 1/250. |
Target
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Function
Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death. -
Tissue specificity
Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated.
Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1. -
Cellular localization
Cytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent. - Information by UniProt
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Database links
- Entrez Gene: 9641 Human
- Omim: 605048 Human
- SwissProt: Q14164 Human
- Unigene: 321045 Human
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Alternative names
- I kappa B kinase epsilon antibody
- I-kappa-B kinase epsilon antibody
- IkBKE antibody
see all
Images
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![Western blot - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)](https://www.abcam.com/ps/products/124/ab124766/Images/ab124766-138622-anti-ikki-ikke-antibody-epr4904-western-blot.jpg)
Western blot - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)All lanes : Anti-IKKi/IKKe antibody [EPR4904] (ab124766) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 80 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)](https://www.abcam.com/ps/products/124/ab124766/Images/ab124766-138621-anti-ikki-ikke-antibody-epr4904-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)ab124766, at 1/100 dilution, staining IKKi/IKKe in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Other - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)](https://www.abcam.com/ps/products/124/ab124766/Images/ab124766-138620-anti-ikki-ikke-antibody-epr4904.jpg)
Other - Anti-IKKi/IKKe antibody [EPR4904] (ab124766)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Yang W et al. Prognostic significance of preoperative IKBKE expression in esophageal squamous cell carcinoma. Onco Targets Ther 11:1305-1314 (2018). IHC-P . Read more (PubMed: 29563809) »
- Harquail J et al. Pax-5 Inhibits NF-?B Activity in Breast Cancer Cells Through IKKe and miRNA-155 Effectors. J Mammary Gland Biol Neoplasia 23:177-187 (2018). Read more (PubMed: 30032344) »
