Anti-IKZF3 antibody (ab88513)

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Western blot - Anti-IKZF3 antibody (ab88513)
  • Western blot - Anti-IKZF3 antibody (ab88513)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody (ab88513)

Key features and details

  • Mouse polyclonal to IKZF3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-IKZF3 antibody
    See all IKZF3 primary antibodies

  • Description

    Mouse polyclonal to IKZF3

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length human IKZF3 (AAH32707.1).

  • Positive control

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab88513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
IHC-P
Use a concentration of 3 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
IHC-P
Use a concentration of 3 µg/ml.

Target

  • Function

    Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.

  • Tissue specificity

    Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.

  • Sequence similarities

    Belongs to the Ikaros C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.

  • Post-translational
    modifications

    Phosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells.

  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • AIO antibody
      • Aiolos antibody
      • IKAROS family zinc finger 3 (Aiolos) antibody
      • IKAROS family zinc finger 3 antibody
      • Ikaros family zinc finger protein 3 antibody
      • IKZF 3 antibody
      • IKZF3 antibody
      • IKZF3_HUMAN antibody
      • zinc finger DNA binding protein Aiolos antibody
      • Zinc finger protein Aiolos antibody
      • Zinc finger protein subfamily 1A 3 (Aiolos) antibody
      • Zinc finger protein subfamily 1A 3 antibody
      • Zinc finger protein subfamily 1A, member 3 antibody
      • ZNFN1A3 antibody
      see all

    Images

    • Western blot - Anti-IKZF3 antibody (ab88513)
      Western blot - Anti-IKZF3 antibody (ab88513)
      Anti-IKZF3 antibody (ab88513) at 1 µg/ml + Jurkat cell lysate at 50 µg

      Predicted band size: 58 kDa
      Observed band size: 58 kDa
      Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-IKZF3 antibody (ab88513)
      Western blot - Anti-IKZF3 antibody (ab88513)
      All lanes : Anti-IKZF3 antibody (ab88513) at 1 µg/ml

      Lane 1 : IKZF3 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 58 kDa
      Observed band size: 58 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody (ab88513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody (ab88513)
      ab88513, at 3 µg/ml, staining IKZF3 in formalin fixed, paraffin embedded human tonsil tissue by Immunoperoxidase/Immunohistochemistry.

    References (0)

    Publishing research using ab88513? Please let us know so that we can cite the reference in this datasheet.

    ab88513 has not yet been referenced specifically in any publications.

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