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- Specific References
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Overview
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Product nameAnti-IKZF3 antibody
See all IKZF3 primary antibodies -
DescriptionMouse polyclonal to IKZF3
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Full length human IKZF3 (AAH32707.1).
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Positive control
- Purchase matching WB positive control:Recombinant Human IKZF3 protein
- WB: Jurkat cell lysate, IK2F3 transfected 293T cell lysate IHC-P: Human tonsil tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab88513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. | |
| IHC-P | Use a concentration of 3 µg/ml. |
Target
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FunctionTranscription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
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Tissue specificityExpressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.
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Sequence similaritiesBelongs to the Ikaros C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells. -
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 22806 Human
- Omim: 606221 Human
- SwissProt: Q9UKT9 Human
- Unigene: 371680 Human
- Unigene: 444388 Human
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Alternative names
- AIO antibody
- Aiolos antibody
- IKAROS family zinc finger 3 (Aiolos) antibody
see all
Images
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Western blot - Anti-IKZF3 antibody (ab88513)Anti-IKZF3 antibody (ab88513) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 58 kDa
Observed band size: 58 kDa
Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-IKZF3 antibody (ab88513)All lanes : Anti-IKZF3 antibody (ab88513) at 1 µg/ml
Lane 1 : IKZF3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody (ab88513)ab88513, at 3 µg/ml, staining IKZF3 in formalin fixed, paraffin embedded human tonsil tissue by Immunoperoxidase/Immunohistochemistry.
References
ab88513 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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