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    ikzf3-antibody-ab88513.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-IKZF3 antibody (ab88513)

  • Datasheet
  • SDS
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Western blot - Anti-IKZF3 antibody (ab88513)
  • Western blot - Anti-IKZF3 antibody (ab88513)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody (ab88513)

Key features and details

  • Mouse polyclonal to IKZF3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human IKZF3 protein (ab132618)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-IKZF3 antibody
    See all IKZF3 primary antibodies
  • Description

    Mouse polyclonal to IKZF3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length human IKZF3 (AAH32707.1).

  • Positive control

    • Purchase matching WB positive control:Recombinant Human IKZF3 protein
    • WB: Jurkat cell lysate, IK2F3 transfected 293T cell lysate IHC-P: Human tonsil tissue
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Recombinant Protein

    • Recombinant Human IKZF3 protein (ab132618)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab88513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
IHC-P
Use a concentration of 3 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
IHC-P
Use a concentration of 3 µg/ml.

Target

  • Function

    Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
  • Tissue specificity

    Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.
  • Sequence similarities

    Belongs to the Ikaros C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession Q9UKT9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 22806 Human
    • Omim: 606221 Human
    • SwissProt: Q9UKT9 Human
    • Unigene: 371680 Human
    • Unigene: 444388 Human
    • Alternative names

      • AIO antibody
      • Aiolos antibody
      • IKAROS family zinc finger 3 (Aiolos) antibody
      • IKAROS family zinc finger 3 antibody
      • Ikaros family zinc finger protein 3 antibody
      • IKZF 3 antibody
      • IKZF3 antibody
      • IKZF3_HUMAN antibody
      • zinc finger DNA binding protein Aiolos antibody
      • Zinc finger protein Aiolos antibody
      • Zinc finger protein subfamily 1A 3 (Aiolos) antibody
      • Zinc finger protein subfamily 1A 3 antibody
      • Zinc finger protein subfamily 1A, member 3 antibody
      • ZNFN1A3 antibody
      see all

    Images

    • Western blot - Anti-IKZF3 antibody (ab88513)
      Western blot - Anti-IKZF3 antibody (ab88513)
      Anti-IKZF3 antibody (ab88513) at 1 µg/ml + Jurkat cell lysate at 50 µg

      Predicted band size: 58 kDa
      Observed band size: 58 kDa
      Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-IKZF3 antibody (ab88513)
      Western blot - Anti-IKZF3 antibody (ab88513)
      All lanes : Anti-IKZF3 antibody (ab88513) at 1 µg/ml

      Lane 1 : IKZF3 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 58 kDa
      Observed band size: 58 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody (ab88513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody (ab88513)
      ab88513, at 3 µg/ml, staining IKZF3 in formalin fixed, paraffin embedded human tonsil tissue by Immunoperoxidase/Immunohistochemistry.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab88513? Please let us know so that we can cite the reference in this datasheet.

    ab88513 has not yet been referenced specifically in any publications.

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