Overview

  • Product name

    Anti-IKZF3 antibody [EPR9342(B)] (HRP)
    See all IKZF3 primary antibodies
  • Description

    Rabbit monoclonal [EPR9342(B)] to IKZF3 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IKZF3 aa 1-100 (N terminal). The exact sequence is proprietary.
    (Peptide available as ab184024)

  • Positive control

    • WB: Raji and Ramos whole cell lysates. IHC-P: normal human spleen tissue sections.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab198961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/7500. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa).Can be blocked with IKZF3 peptide (ab184024).

Can be blocked with ab184024

Target

  • Function

    Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
  • Tissue specificity

    Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.
  • Sequence similarities

    Belongs to the Ikaros C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AIO antibody
    • Aiolos antibody
    • IKAROS family zinc finger 3 (Aiolos) antibody
    • IKAROS family zinc finger 3 antibody
    • Ikaros family zinc finger protein 3 antibody
    • IKZF 3 antibody
    • IKZF3 antibody
    • IKZF3_HUMAN antibody
    • zinc finger DNA binding protein Aiolos antibody
    • Zinc finger protein Aiolos antibody
    • Zinc finger protein subfamily 1A 3 (Aiolos) antibody
    • Zinc finger protein subfamily 1A 3 antibody
    • Zinc finger protein subfamily 1A, member 3 antibody
    • ZNFN1A3 antibody
    see all

Images

  • All lanes : Anti-IKZF3 antibody [EPR9342(B)] (HRP) (ab198961) at 1/7500 dilution

    Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 90 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198961 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of IKZF3 staining in a section of formalin-fixed paraffin-embedded normal human spleen tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198961 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

References

ab198961 has not yet been referenced specifically in any publications.

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