Overview

  • Product name

    Anti-IKZF3 antibody [EPR9343(B)]
    See all IKZF3 primary antibodies
  • Description

    Rabbit monoclonal [EPR9343(B)] to IKZF3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control

    • Raji and Ramos lysate; Human spleen tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab139182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
    • Tissue specificity

      Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.
    • Sequence similarities

      Belongs to the Ikaros C2H2-type zinc-finger protein family.
      Contains 6 C2H2-type zinc fingers.
    • Post-translational
      modifications

      Phosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells.
    • Cellular localization

      Nucleus. Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • AIO antibody
      • Aiolos antibody
      • IKAROS family zinc finger 3 (Aiolos) antibody
      • IKAROS family zinc finger 3 antibody
      • Ikaros family zinc finger protein 3 antibody
      • IKZF 3 antibody
      • IKZF3 antibody
      • IKZF3_HUMAN antibody
      • zinc finger DNA binding protein Aiolos antibody
      • Zinc finger protein Aiolos antibody
      • Zinc finger protein subfamily 1A 3 (Aiolos) antibody
      • Zinc finger protein subfamily 1A 3 antibody
      • Zinc finger protein subfamily 1A, member 3 antibody
      • ZNFN1A3 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling IKZF3 with ab139182 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-IKZF3 antibody [EPR9343(B)] (ab139182) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 58 kDa

    References

    ab139182 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab139182.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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