Recombinant
RabMAb

Recombinant Anti-IKZF3 antibody [EPR9343(B)] - BSA and Azide free (ab224283)

Overview

  • Product name

    Anti-IKZF3 antibody [EPR9343(B)] - BSA and Azide free
    See all IKZF3 primary antibodies
  • Description

    Rabbit monoclonal [EPR9343(B)] to IKZF3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human IKZF3 aa 400-500. The exact sequence is proprietary.

  • Positive control

    • Raji and Ramos lysate; Human spleen tissue.
  • General notes

    Ab224283 is the carrier-free version of ab139182. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab224283 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab224283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
    • Tissue specificity

      Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.
    • Sequence similarities

      Belongs to the Ikaros C2H2-type zinc-finger protein family.
      Contains 6 C2H2-type zinc fingers.
    • Post-translational
      modifications

      Phosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells.
    • Cellular localization

      Nucleus. Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • AIO antibody
      • Aiolos antibody
      • IKAROS family zinc finger 3 (Aiolos) antibody
      • IKAROS family zinc finger 3 antibody
      • Ikaros family zinc finger protein 3 antibody
      • IKZF 3 antibody
      • IKZF3 antibody
      • IKZF3_HUMAN antibody
      • zinc finger DNA binding protein Aiolos antibody
      • Zinc finger protein Aiolos antibody
      • Zinc finger protein subfamily 1A 3 (Aiolos) antibody
      • Zinc finger protein subfamily 1A 3 antibody
      • Zinc finger protein subfamily 1A, member 3 antibody
      • ZNFN1A3 antibody
      see all

    References

    ab224283 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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