Key features and details
- Rabbit polyclonal to IKZF4
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-IKZF4 antibody
See all IKZF4 primary antibodies
DescriptionRabbit polyclonal to IKZF4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Synthetic peptide within Mouse IKZF4 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q8C208
- This antibody gave a positive signal in both Mouse and Rat Brain tissue lysates.
This product was previously labelled as ZNFN1A4
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).|
FunctionDNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems.
Tissue specificityHighly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K562. Highly expressed in THP-1 and Mo7e-IL3 cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.
Sequence similaritiesBelongs to the Ikaros C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers.
DomainThe N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.
C-terminal zinc fingers mediate homodimerization.
- Information by UniProt
- EOS antibody
- Eos protein antibody
- IKAROS family zinc finger 4 (Eos) antibody
All lanes : Anti-IKZF4 antibody (ab155754) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)
Exposure time: 16 minutes
Please note that Mouse Liver tissue lysate in lane 3 represents a negative control for IKZF4.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab155754 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
ab155754 has not yet been referenced specifically in any publications.