Overview

  • Product name

  • Description

    Rabbit polyclonal to IKZF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse IKZF4 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q8C208

  • Positive control

    • This antibody gave a positive signal in both Mouse and Rat Brain tissue lysates.
  • General notes

     This product was previously labelled as ZNFN1A4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).

Target

  • Function

    DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems.
  • Tissue specificity

    Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K562. Highly expressed in THP-1 and Mo7e-IL3 cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.
  • Sequence similarities

    Belongs to the Ikaros C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • Domain

    The N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.
    C-terminal zinc fingers mediate homodimerization.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • EOS antibody
    • Eos protein antibody
    • IKAROS family zinc finger 4 (Eos) antibody
    • IKAROS family zinc finger 4 antibody
    • Ikaros family zinc finger protein 4 antibody
    • IKZF4 antibody
    • IKZF4_HUMAN antibody
    • KIAA1782 antibody
    • Zinc finger protein Eos (IKAROS family zinc finger protein 4) antibody
    • Zinc finger protein Eos antibody
    • Zinc finger protein, subfamily 1A, 4 (Eos) antibody
    • Zinc finger protein, subfamily 1A, member 4 antibody
    • Zinc finger transcription factor Eos antibody
    • ZNFN1A4 antibody
    see all

Images

  • All lanes : Anti-IKZF4 antibody (ab155754) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 64 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 200 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)


    Exposure time: 16 minutes


    Please note that Mouse Liver tissue lysate in lane 3 represents a negative control for IKZF4.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab155754 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

References

ab155754 has not yet been referenced specifically in any publications.

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