Key features and details
- Rabbit polyclonal to IKZF4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IKZF4 antibody
See all IKZF4 primary antibodies
DescriptionRabbit polyclonal to IKZF4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human IKZF4 aa 268-457. The exact sequence is proprietary.
Database link: Q9H2S9
- Raji and K562 whole cell lysates; U373 xenograft tissue.
This product was previously labelled as ZNFN1A4
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 64 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionDNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems.
Tissue specificityHighly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K562. Highly expressed in THP-1 and Mo7e-IL3 cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.
Sequence similaritiesBelongs to the Ikaros C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers.
DomainThe N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.
C-terminal zinc fingers mediate homodimerization.
- Information by UniProt
- EOS antibody
- Eos protein antibody
- IKAROS family zinc finger 4 (Eos) antibody
All lanes : Anti-IKZF4 antibody (ab183625) at 1/1000 dilution
Lane 1 : Raji whole cell lysate
Lane 2 : K562 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 64 kDa
7.5 % SDS-PAGE
Immunohistochemical analysis of paraffin-embedded U373 xenograft tissue labeling IKZF4 with ab183625 at 1/500 dilution.
ab183625 has not yet been referenced specifically in any publications.