Overview

  • Product name

  • Description

    Rabbit polyclonal to IKZF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IKZF4 aa 268-457. The exact sequence is proprietary.
    Database link: Q9H2S9

  • Positive control

    • Raji and K562 whole cell lysates; U373 xenograft tissue.
  • General notes

     This product was previously labelled as ZNFN1A4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 64 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems.
  • Tissue specificity

    Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K562. Highly expressed in THP-1 and Mo7e-IL3 cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.
  • Sequence similarities

    Belongs to the Ikaros C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • Domain

    The N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.
    C-terminal zinc fingers mediate homodimerization.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • EOS antibody
    • Eos protein antibody
    • IKAROS family zinc finger 4 (Eos) antibody
    • IKAROS family zinc finger 4 antibody
    • Ikaros family zinc finger protein 4 antibody
    • IKZF4 antibody
    • IKZF4_HUMAN antibody
    • KIAA1782 antibody
    • Zinc finger protein Eos (IKAROS family zinc finger protein 4) antibody
    • Zinc finger protein Eos antibody
    • Zinc finger protein, subfamily 1A, 4 (Eos) antibody
    • Zinc finger protein, subfamily 1A, member 4 antibody
    • Zinc finger transcription factor Eos antibody
    • ZNFN1A4 antibody
    see all

Images

  • All lanes : Anti-IKZF4 antibody (ab183625) at 1/1000 dilution

    Lane 1 : Raji whole cell lysate
    Lane 2 : K562 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 64 kDa



    7.5 % SDS-PAGE

  • Immunohistochemical analysis of paraffin-embedded U373 xenograft tissue labeling IKZF4 with ab183625 at 1/500 dilution.

References

ab183625 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183625.
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