Overview

  • Product name

    Anti-IKZF4 antibody [EPR11804]
    See all IKZF4 primary antibodies
  • Description

    Rabbit monoclonal [EPR11804] to IKZF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human IKZF4. The exact sequence is proprietary.
    Database link: Q9H2S9

  • Positive control

    • HeLa, HepG2, THP1 and Human skeletal muscle lysates; HeLa and THP1 cells.
  • General notes

     This product was previously labelled as ZNFN1A4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11804
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 64 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems.
    • Tissue specificity

      Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K562. Highly expressed in THP-1 and Mo7e-IL3 cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.
    • Sequence similarities

      Belongs to the Ikaros C2H2-type zinc-finger protein family.
      Contains 6 C2H2-type zinc fingers.
    • Domain

      The N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.
      C-terminal zinc fingers mediate homodimerization.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • EOS antibody
      • Eos protein antibody
      • IKAROS family zinc finger 4 (Eos) antibody
      • IKAROS family zinc finger 4 antibody
      • Ikaros family zinc finger protein 4 antibody
      • IKZF4 antibody
      • IKZF4_HUMAN antibody
      • KIAA1782 antibody
      • Zinc finger protein Eos (IKAROS family zinc finger protein 4) antibody
      • Zinc finger protein Eos antibody
      • Zinc finger protein, subfamily 1A, 4 (Eos) antibody
      • Zinc finger protein, subfamily 1A, member 4 antibody
      • Zinc finger transcription factor Eos antibody
      • ZNFN1A4 antibody
      see all

    Images

    • Western blot analysis on Immunoprecipitation pellet from HeLa cell lysate showing IKZF4 , using ab170957 at 1/10 dilution for IP.

    • All lanes : Anti-IKZF4 antibody [EPR11804] (ab170957) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Human skeletal lysate
      Lane 4 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 64 kDa

    References

    ab170957 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170957.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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