Key features and details
- Rabbit polyclonal to IL-1 alpha
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-1 alpha antibody
See all IL-1 alpha primary antibodies
DescriptionRabbit polyclonal to IL-1 alpha
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human IL-1 alpha (internal sequence). The exact sequence is proprietary.
Database link: P01583
- IHC-P: Human colon carcinoma tissue. WB: HepG2 whole cell lysate. ICC/IF: NCI-H1299 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 31 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Sequence similaritiesBelongs to the IL-1 family.
DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
- Information by UniProt
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
Paraffin-embedded human colon carcinoma tissue stained for IL-1 alpha using ab227482 at 1/250 dilution in immunohistochemical analysis.
Anti-IL-1 alpha antibody (ab227482) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 31 kDa
12% SDS-PAGE gel.
4% paraformaldehyde-fixed NCI-H1299 (human lung carcinoma cell line) cells stained for IL-1 alpha (green) using ab227482 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227482 has been referenced in 1 publication.
- Bian ZM et al. Expression and regulation of alarmin cytokine IL-1a in human retinal pigment epithelial cells. Exp Eye Res 172:10-20 (2018). Human . PubMed: 29551335