Product nameAnti-IL-1 alpha antibody
See all IL-1 alpha primary antibodies
DescriptionRabbit polyclonal to IL-1 alpha
Tested applicationsSuitable for: ELISA, Flow Cyt, IHC-Fr, Neutralising, WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Non human primates
Recombinant full length protein corresponding to Human IL-1 alpha.
- Purchase matching WB positive control:Recombinant human IL-1 alpha protein
- Supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC)(PBMC stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification noteswhole rabbit serum purified by DEAE fractionation
Our Abpromise guarantee covers the use of ab7632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/200 - 1/1000.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/100 - 1/200. Detects a band of approximately 17,31 kDa.|
|IHC-P||Use at an assay dependent concentration. PubMed: 22190968|
FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Sequence similaritiesBelongs to the IL-1 family.
DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
- Information by UniProt
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
ab7632 staining IL-1 alpha in murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 4°C and then incubated with ab7632 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/500 dilution.
Immunohistochemical analysis of pancreatic ductal adenocarcinoma tissue, staining IL-1 alpha with ab7632.
Antigen retrieval was performed by heat mediation in citrate buffer (pH 6.0). Tissue was blocked with 1% BSA for 10 min. The samples incubated overnight with primary antibody (1/40). Staining was detected using DAB. Sections were counterstained with hematoxylin.
This product has been referenced in:
- Singh AK et al. Critical role of IL-1a in IL-1ß-induced inflammatory responses: cooperation with NF-?Bp65 in transcriptional regulation. FASEB J 33:2526-2536 (2019). Read more (PubMed: 30272996) »
- Burzynski LC et al. The Coagulation and Immune Systems Are Directly Linked through the Activation of Interleukin-1a by Thrombin. Immunity 50:1033-1042.e6 (2019). Read more (PubMed: 30926232) »