Application
Flow Cytometry
Sample
Human Cell (Platelets)
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy
Platelets
Specification
Platelets
Preparation
Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Sample buffer: PBS
Fixation
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Flour488 Chicken Anti Rabbit IgG (H+L)
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Abbreviations in Fig
P : Permeabilized
US : Unstained (Red Peak)
IGG RB : IgG Rabbit (Blue Peak)
IL1A : IL1 alpha Ab (Green Peak)
Abcam response
Shorter incubation with the primary might reduce non-specific binding.
Dr. Mahesh Shivananjappa
Verified customer
Submitted Dec 22 2010