Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (immortalised bone marrow-derived macrophages)
Permeabilization
Yes - 0.1% Triton X-100
Specification
immortalised bone marrow-derived macrophages
Blocking step
Fish gelatine buffer with 0.1% casein and 5% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: blocking buffer
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa-fluor 568 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Abcam response
Thank you for submitting this Abreview. For immunocytochemistry, we recommend a 1/100 dilution, which may improve the signal.
DR. James Harris
Verified customer
Submitted Sep 08 2014