Anti-IL-1 alpha antibody (ab9614)
Key features and details
- Rabbit polyclonal to IL-1 alpha
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Isotype: unknown
Overview
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Product name
Anti-IL-1 alpha antibody
See all IL-1 alpha primary antibodies -
Description
Rabbit polyclonal to IL-1 alpha -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Highly pure (>98%) recombinant hIL-1a (human Interleukin-1-alpha).
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Positive control
- Purchase matching WB positive control:Recombinant human IL-1 alpha protein
- Recombinant human IL-1 alpha protein (ab9615) can be used as a positive control in WB.
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab7632
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
No preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab9614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. To detect IL-1 αlpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271226 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-1 αlpha. |
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| WB | Use at an assay dependent concentration. To detect hIL-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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Function
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
Sequence similarities
Belongs to the IL-1 family. -
Domain
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
Cellular localization
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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Database links
- Entrez Gene: 3552 Human
- Omim: 147760 Human
- SwissProt: P01583 Human
- Unigene: 1722 Human
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Alternative names
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
see all
Images
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Western blot - Anti-IL-1 alpha antibody (ab9614) -
Sandwich ELISA - Anti-IL-1 alpha antibody (ab9614)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (13)
ab9614 has been referenced in 13 publications.
- Lau L et al. Uncoupling the Senescence-Associated Secretory Phenotype from Cell Cycle Exit via Interleukin-1 Inactivation Unveils Its Protumorigenic Role. Mol Cell Biol 39:N/A (2019). PubMed: 30988157
- Rajan A et al. Impact of Nuclear Interleukin-1 Alpha and EGFR Expression on Recurrence and Survival Outcomes in Oral Squamous Cell Carcinomas. J Oncol 2019:5859680 (2019). PubMed: 31320902
- Yang L et al. miRNA-544a Regulates the Inflammation of Spinal Cord Injury by Inhibiting the Expression of NEUROD4. Cell Physiol Biochem 51:1921-1931 (2018). PubMed: 30513512
- Rawat P et al. Human immunodeficiency virus Type-1 single-stranded RNA activates the NLRP3 inflammasome and impairs autophagic clearance of damaged mitochondria in human microglia. Glia N/A:N/A (2018). PubMed: 30582668
- Folco EJ et al. Neutrophil Extracellular Traps Induce Endothelial Cell Activation and Tissue Factor Production Through Interleukin-1a and Cathepsin G. Arterioscler Thromb Vasc Biol 38:1901-1912 (2018). PubMed: 29976772
