Anti-IL-1 alpha antibody (ab9614)
Rabbit polyclonal IL-1 alpha antibody. Validated in WB, ELISA, IHC, Neut, ICC/IF and tested in Human. Cited in 8 publication(s). Independently reviewed in 3 review(s).
- Datasheet
- References (8)
- Protocols
Overview
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Product name
Anti-IL-1 alpha antibody
See all IL-1 alpha primary antibodies -
Description
Rabbit polyclonal to IL-1 alpha -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ELISA, Neutralising, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Highly pure (>98%) recombinant hIL-1a (human Interleukin-1-alpha).
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Positive control
- Purchase matching WB positive control:Recombinant human IL-1 alpha protein
- Recombinant human IL-1 alpha protein (ab9615) can be used as a positive control in WB.
Properties
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Form
Lyophilised:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
PBS, pH 7.4, no preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab9614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. To detect hIL-1a by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-1a. | |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1a (20 ng/ml), a concentration of 1.0 - 2.0 µg/ml of this antibody is required. | |
| WB | Use at an assay dependent concentration. To detect hIL-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Incubate overnight, at 4oC. |
Target
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Function
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
Sequence similarities
Belongs to the IL-1 family. -
Domain
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
Cellular localization
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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Database links
- Entrez Gene: 3552 Human
- Omim: 147760 Human
- SwissProt: P01583 Human
- Unigene: 1722 Human
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Alternative names
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody (ab9614)ab9614 at 1.0 µg/ml staining normal human skin by IHC-P. The antibody was incubated overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen. Enzyme induced antigen retrieval was performed with proteinase K. -
Immunocytochemistry/ Immunofluorescence - Anti-IL-1 alpha antibody (ab9614)This image is courtesy of an Abreview submitted by Mhairi Morrisab9614 at 1/50 staining IL-1 in human primary tonsillar epithelial cell by immunocytochemistry/ immunofluorescence. The cells were paraformaldehyde fixed and blocked with 20% serum prior to incubation with the antibody for 1 hour. Alexa fluor® 594 goat polyclonal to rabbit Ig, diluted 1/1000, was used as the secondary antibody.
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Western blot - Anti-IL-1 alpha antibody (ab9614)
Protocols
References
This product has been referenced in:
- Takahashi T et al. Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma. J Exp Med 214:1129-1151 (2017). Read more (PubMed: 28232470) »
- Wang S et al. Centromere protein U is a potential target for gene therapy of human bladder cancer. Oncol Rep 38:735-744 (2017). Read more (PubMed: 28677729) »
