Key features and details
- Goat polyclonal to IL-1 alpha
- Suitable for: WB, Sandwich ELISA, Neutralising
- Reacts with: Rat
- Isotype: unknown
Product nameAnti-IL-1 alpha antibody
See all IL-1 alpha primary antibodies
DescriptionGoat polyclonal to IL-1 alpha
Tested applicationsSuitable for: WB, Sandwich ELISA, Neutralisingmore details
Species reactivityReacts with: Rat
Highly pure (>98%) recombinant rIL-1a (rat Interleukin-1-alpha).
- WB: Recombinant rat IL-1 alpha protein (ab9877).
Although some customers have been successful in IHC we no longer batch test in this application.
FormLyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
No preservative, sterile filtered
Concentration information loading...
PurityImmunogen affinity purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to IL1 alpha (Biotin) (ab84012). Using ab84012 as the detection antibody and ab109792 as the capture antibody, the detection limit for recombinant Rat IL1 alpha is 0.2 - 0.4 ng/well|
|Neutralising||Use a concentration of 0.011 - 0.017 µg/ml. Yields one-half maximal inhibition [ND50] of the biological activity of Rat IL1 alpha (50 pg/ml).|
FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Sequence similaritiesBelongs to the IL-1 family.
DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
- Information by UniProt
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab9875 has been referenced in 2 publications.
- Vasudeva K et al. In vivo and systems biology studies implicate IL-18 as a central mediator in chronic pain. J Neuroimmunol 283:43-9 (2015). PubMed: 26004155
- Johnson EA & Kan RK The acute phase response and soman-induced status epilepticus: temporal, regional and cellular changes in rat brain cytokine concentrations. J Neuroinflammation 7:40 (2010). IHC-FoFr ; Rat . PubMed: 20649973