Overview

  • Product name
    Anti-IL-1 alpha antibody [AS5]
    See all IL-1 alpha primary antibodies
  • Description
    Mouse monoclonal [AS5] to IL-1 alpha
  • Host species
    Mouse
  • Specificity
    Recognizes human IL-1 alpha. Does not recognize mouse IL-1 alpha, human IL-1 beta or a panel of other human cytokines.
  • Tested applications
    Suitable for: IHC-P, IP, ELISA, Neutralising, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IL-1 alpha.

Properties

Applications

Our Abpromise guarantee covers the use of ab17281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. (see Abreview)
IP Use at an assay dependent concentration. This antibody has been used to immunoprecipitate >90% human IL-1 (in solution using a double-antibody method).
ELISA Use a concentration of 2 µg/ml. This antibody has been used as a solution phase antibody in a two site ELISA with biotin conjugated IL-1 alpha antibody. Typically the ELISA has a working range of 0-10000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of human IL-1 alpha in human serum is typically >85% in the ELISA.
Neutralising Use a concentration of 25 mg/ml. This antibody has been used to neutralize >95% of the biological activity of human IL-1 at 0.5 mg/ml.
IHC (PFA fixed) Use a concentration of 1 - 10 µg/ml. This antibody has been used to stain human IL-1 alpha expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10 minutes at 4oC plus 0.1% saponin 20 minutes at RT.

Target

  • Function
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similarities
    Belongs to the IL-1 family.
  • Domain
    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localization
    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAF antibody
    • FAF antibody
    • Hematopoietin 1 antibody
    • Hematopoietin-1 antibody
    • IL 1 alpha antibody
    • IL 1A antibody
    • IL-1 alpha antibody
    • Il-1a antibody
    • IL1 ALPHA antibody
    • IL1 antibody
    • IL1A antibody
    • IL1A_HUMAN antibody
    • IL1F1 antibody
    • Interleukin 1 alpha antibody
    • Interleukin-1 alpha antibody
    • Interleukin1 alpha antibody
    • LAF antibody
    • LEM antibody
    • Preinterleukin 1 alpha antibody
    • Pro interleukin 1 alpha antibody
    see all

Images

  • ab17281 staining IL1 alpah in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using EDTA buffer pH 9.0. Samples were then blocked with 10% serum for 30 minutes at 21°C followed by incubation with the primary antibody at a 1/200 dilution for 30 minutes at 21°C. A undiluted HRP-conjugated goat polyclonal was used as secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Zhang X  et al. Expression of TP53 and IL-1a in unicystic ameloblastoma predicts the efficacy of marsupialization treatment. Medicine (Baltimore) 97:e9795 (2018). Read more (PubMed: 29419674) »
  • Zhang X  et al. H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis. Nat Commun 8:14799 (2017). Read more (PubMed: 28300060) »
See all 2 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

We look forward to your review. If the results are still negative, please let us know. We welcome negative reviews as well as positive ones. If the signal from the patient samples you plan to test are also faint or negative, compared to the normal tissue you have been testing the antibody with, that is useful information for customers trying to choose among the IL1 antibodies we offer.

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Question
Answer

Thanks for your enquiry.

Its estimated we will havemore vialsof ab17281 in stock by Monday 6, 2012.

I hope this is helpful. Please contact us again if you have any further questions.

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Question
Answer

Thanks for the reply.

A new vial of ab17281 is on its way to you, Your new order number if *.

If there is anything else I can help you with, or if you have any issues with the new vial of antibody, then please let me know.

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Answer

Thank you for your reply.

Unfortunately we do not have any other lots of this antibody available, so maybe it would be better to send a different IL-1 alpha antibody for you try.Or if you wish to give ab17281 another try, I’m happy to send you another vial of that.

I look forward to your reply and helping to resolve this issue.

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Question
Answer

Thanks for letting me know.

I am sorry that increasing the primary antibody concentration did not work for you. Therefore I think the best way to move forward on this one, is to either send you a new aliquot of ab17281, in case there was something wrong with the vial we sent, or I could send you a different antibody against the same target, ab7632 perhaps:

https://www.abcam.com/IL1-alpha-antibody-ab7632.html



Please let me know how you would like to continue and if you could also include either the Abcam order number or the PO# for ab17281 in the reply that would be very helpful.

I look forward to your reply.

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Question
Answer

Thank you for contacting Abcam.

I am sorry about the issues you have been having with ab17281 in IHC-P. If the protocol variations we talked about today do not prove to be successful, then please let me know as the antibody is covered under our Abpromise to work in IH-P on human samples.

I hope all is going well.

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Question

Product Information Product Name: IL1 alpha antibody Cat. # ab17281 Lot No: GR36829-1 Ordered:June 2011 PO Number: 062111AC_Rev 1. Order details: • Antibody storage conditions (temperature/reconstitution etc) -20℃ 2. Please describe the problem (high background, no staining etc). No staining 3. On what material are you testing the antibody in IHC • Species: Human • Cell Line or tissue: Tissue 4. How did you fix the samples? • Ethanol, methanol: (was it ice cold) • Acetone: (was it ice cold) • Paraformaldehyde or formalin (percentage, perfusion fixed on not): formalin • Fixation time:12h • Fixation temperature: room temperature • Permeabilization ( reagents, time and incubation temperature): 5. For formalin-fixed paraffin embedded tissue: did you apply antigen retrieval step? • Enzymatic method( reagents, time and incubation temperature): • Heat mediated technique( buffers, time and incubation temperature): EDTA, high pressure,120℃,2-3min citric acid, microwave • Other : 6. Blocking steps: • For HRP detection method: did block endogenous peroxidases • How did you block the unspecific binding sites: serum, BSA, Milk, other: serum • For how long: Length of 10min 7. Primary antibody • Specification (in which species was it raised against): Human • At what dilution(s) have you tested this antibody: 1:50.1:100 • What dilution buffer was used: PBS • Incubation time:40min/ over night • Incubation temperature:37℃/4℃ • What washing steps were done: 8 times 8. Secondary antibody • Specification (in which species was it raised against): Anti-Mouse • At what dilution(s) have you tested this antibody: Pre-formed • What dilution buffer was used: • Incubation time: 30min • Incubation temperature: 37℃ • What washing steps were done: 5 times (5 times the steps or washed 5 times?) • Do you know whether the problems you are experiencing come from the secondary? 9. What detection method are you using? DAB 10. Background staining • Please provide an image of your staining 11. Which detection system did you use? 12. Did you apply positive and negative controls along with the samples? Please specify. Yes, Human Liver 13. Optimization attempts • How many times have you tried the IHC? Over 10 times • Do you obtain the same results every time? Yes • What steps have you altered? Primary antibody dilution, incubation time and temperature, antigen retrieval steps

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Answer

Thank you once again for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. Reviewing this case, I would like to offer some suggestions to help optimise the results from ab17281. I would also appreciate if you can confirm some further details: 1. For antigen retreival, I can suggest to continue to try heat mediated retreival with EDTA pH9, as this has been used succesfully in an Abreview submitted by another customer (published on the datasheet). However, I woudl recommend to try different time points as this may help to optmize the results. Try heating for 1, 3,5, 10 and 20 minutes. 2. I can recommend to try incubating in PBS containing 0.2% Tween. The Tween will help to solubilise the antibody. It will also help to permeablize the cells to allow staining of any IL1 alpha in the cytoplasm. 3. PBS continaing 0.2% tween can also be used as a wash buffer. Wash 3 times only at each appropriate wash step. 4. Could you confirm if the current vial of secondary antibody has been used succesfully with other primary antibodies? In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Specification
Colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH 9.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C

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Verified customer

Submitted Jan 11 2011

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