FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferConstituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 31 kDa.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Sequence similaritiesBelongs to the IL-1 family.
DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
- Information by UniProt
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
All lanes : Anti-IL-1 alpha antibody (Biotin) (ab271258) at 0.2 µg/ml
Lane 1 : Recombinant Mouse IL-1 alpha protein at 0.25 µg
Lane 2 : Recombinant Mouse IL-1 alpha protein at 0.125 µg
Lane 3 : Recombinant Mouse IL-1 alpha protein at 0.0625 µg
Lane 4 : Recombinant Mouse IL-1 alpha protein at 0.03125 µg
Lane 5 : Recombinant Mouse IL-1 alpha protein at 0.015625 µg
Lane 6 : Recombinant Mouse IL-1 alpha protein at 0.0078 µg
Lane 7 : Recombinant Mouse IL-1 alpha protein at 0.0039 µg
Lane 8 : Recombinant Mouse IL-1 alpha protein at 0.00195 µg
Lane 9 : Recombinant Mouse IL-1 alpha protein at 0.000975 µg
Lane 10 : Recombinant Mouse IL-1 alpha protein at 0.0004875 µg
Lane 11 : Recombinant Mouse IL-1 alpha protein at 0.00024 µg
Predicted band size: 31 kDa
Mouse IL-1 alpha was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271258. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mouse IL-1 alpha protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271258 has not yet been referenced specifically in any publications.