Key features and details
- Goat polyclonal to IL-1 alpha (Biotin)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-IL-1 alpha antibody (Biotin)
See all IL-1 alpha primary antibodies
DescriptionGoat polyclonal to IL-1 alpha (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Rat
Recombinant full length protein corresponding to Rat IL-1 alpha.
General notesab84012 is sterile filtered.
FormLyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab84012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat IL-1 alpha.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 31 kDa.
The detection limit for recombinant rat IL-1 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Sequence similaritiesBelongs to the IL-1 family.
DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
- Information by UniProt
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab84012 has not yet been referenced specifically in any publications.