Recombinant Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)
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- References
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Overview
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Product name
Anti-IL-1 alpha antibody [EPR19904-247]
See all IL-1 alpha primary antibodies -
Description
Rabbit monoclonal [EPR19904-247] to IL-1 alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human IL-1 alpha aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: P01583 -
Positive control
- WB: Human IL-1 alpha recombinant protein (aa113-271); THP-1 treated with 50 nM phorbol-12-myristate-13-acetate (PMA) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, cell lysate. Flow Cyt: THP-1 treated with 50 nM phorbol-12-myristate-13-acetate (PMA) and 5 µg/ml lipopolysaccharide (LPS) for 24 hours.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19904-247 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab206410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 34, 20, 15 kDa (predicted molecular weight: 31 kDa). | |
| Flow Cyt | 1/60. |
Target
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Function
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
Sequence similarities
Belongs to the IL-1 family. -
Domain
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
Cellular localization
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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Database links
- Entrez Gene: 3552 Human
- Omim: 147760 Human
- SwissProt: P01583 Human
- Unigene: 1722 Human
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Alternative names
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
see all
Images
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![Flow Cytometry - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)](https://www.abcam.com/ps/products/206/ab206410/Images/ab206410-314415-anti-il-1-alpha-antibody-epr19904-247-flow-cytometry.jpg)
Flow Cytometry - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (human monocytic leukemia cell line) cell line treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 μg/ml lipopolysaccharide (LPS) for 24 hours (red) or an untreated control (green) labeling IL-1 alpha with ab206410 at 1/60 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)](https://www.abcam.com/ps/products/206/ab206410/Images/ab206410-314414-anti-il-1-alpha-antibody-epr19904-247-western-blot.jpg)
Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)All lanes : Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution
Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1, treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 15,20,34 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Expression of IL-1 alpha can be induced by LPS in macrophages. The molecular masses observed are consistent with the literature (PMID: 9785472; PMID: 1868253; PMID:19104081).
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![Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)](https://www.abcam.com/ps/products/206/ab206410/Images/ab206410-314413-anti-il-1-alpha-antibody-epr19904-247-western-blot.jpg)
Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution + Human IL-1 alpha recombinant protein (aa113-271) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
References
ab206410 has not yet been referenced specifically in any publications.
