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    il-1-alpha-antibody-epr19904-247-ab206410.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)

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  • Certificate of Compliance
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Flow Cytometry - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)
  • Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)
  • Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)
  • Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19904-247] to IL-1 alpha
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
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Human IL-1a ELISA Kit (ab178008)

View more associated products

Overview

  • Product name

    Anti-IL-1 alpha antibody [EPR19904-247]
    See all IL-1 alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR19904-247] to IL-1 alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IL-1 alpha aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P01583

  • Positive control

    • WB: Human IL-1 alpha recombinant protein (aa113-271); THP-1 treated with 50 nM phorbol-12-myristate-13-acetate (PMA) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, cell lysate. Flow Cyt: THP-1 treated with 50 nM phorbol-12-myristate-13-acetate (PMA) and 5 µg/ml lipopolysaccharide (LPS) for 24 hours.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19904-247
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Immunology
    • Secreted Molecules
    • Other secreted molecules
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free (ab237037)
    • Anti-IL-1 alpha antibody [EPR19904-247] - Low endotoxin, Azide free (ab246706)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human IL-1 alpha protein (ab9615)
  • Related Products

    • Phorbol 12-myristate 13-acetate (PMA), PKC activator (ab120297)

Applications

Our Abpromise guarantee covers the use of ab206410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 34, 20, 15 kDa (predicted molecular weight: 31 kDa).
Flow Cyt 1/60.

Target

  • Function

    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similarities

    Belongs to the IL-1 family.
  • Domain

    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localization

    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Target information above from: UniProt accession P01583 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3552 Human
    • Omim: 147760 Human
    • SwissProt: P01583 Human
    • Unigene: 1722 Human
    • Alternative names

      • BAF antibody
      • FAF antibody
      • Hematopoietin 1 antibody
      • Hematopoietin-1 antibody
      • IL 1 alpha antibody
      • IL 1A antibody
      • IL-1 alpha antibody
      • Il-1a antibody
      • IL1 ALPHA antibody
      • IL1 antibody
      • IL1A antibody
      • IL1A_HUMAN antibody
      • IL1F1 antibody
      • Interleukin 1 alpha antibody
      • Interleukin-1 alpha antibody
      • Interleukin1 alpha antibody
      • LAF antibody
      • LEM antibody
      • Preinterleukin 1 alpha antibody
      • Pro interleukin 1 alpha antibody
      see all

    Images

    • Flow Cytometry - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)
      Flow Cytometry - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (human monocytic leukemia cell line) cell line treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 μg/ml lipopolysaccharide (LPS) for 24 hours (red) or an untreated control (green) labeling IL-1 alpha with ab206410 at 1/60 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)
      Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)
      All lanes : Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution

      Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
      Lane 2 : THP-1, treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 31 kDa
      Observed band size: 15,20,34 kDa
      why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Expression of IL-1 alpha can be induced by LPS in macrophages. The molecular masses observed are consistent with the literature (PMID: 9785472; PMID: 1868253; PMID:19104081). 

    • Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)
      Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)
      Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution + Human IL-1 alpha recombinant protein (aa113-271) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 31 kDa


      Exposure time: 3 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)
      Anti-IL-1 alpha antibody [EPR19904-247] (ab206410)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab206410? Please let us know so that we can cite the reference in this datasheet.

    ab206410 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab206410.
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