Flow Cytometry abreview for Anti-IL-1 beta antibody

Good
Abreviews
Application
Flow Cytometry
Sample
Human Cell (human monocytes)
Permeabilization
Yes - 0,1% Saponin / PBS
Gating Strategy
no gate
Specification
human monocytes
Preparation
Cell harvesting/tissue preparation method: isolated by gradient centrifugation and followed by magnetic isolation
Sample buffer: 0,1% Saponin /1% FCS/ PBS
Fixation
Paraformaldehyde

Other product details

Incubation time
45 minute(s) · Temperature: 4°C · Diluent: 0,1% Saponin /1% FCS/ PBS
Dilution
1/500

Secondary antibody

Dilution
1/100
Name
Non-Abcam antibody was used: goat anti-rabbit Alexa488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
1 = unstimulated Monocytes; 2 = Monocytes stimulated with LPS.

Abcam user community

Verified customer

Submitted Oct 08 2013

Sign up