Western blot abreview for Anti-IL-1 beta antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (peripheral blood mononuclear cells)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gel)
Loading amount
25 µg
Treatment
see below
Specification
peripheral blood mononuclear cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (5%BSA in TBST)
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
chemiluminescence
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: ~35 kDa
Positive control
rh IL-1beta protein

Additional data

Additional Notes
lanes: 1= PBMC`s unstimulated 2 = Monocytes unstimualted 3 = Monocytes stimulated with LPS 4 = positive control (rh IL-1beta protein)

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Submitted Oct 07 2013

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