Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
Yes - 0,1% Saponin/PBS
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/50

Secondary antibody

Concentration
10 µg/ml
Name
Non-Abcam antibody was used: goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

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Submitted Oct 11 2013

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