Western blot abreview for Anti-IL-1 beta antibody

Average
Abreviews
Application
Western blot
Sample
Cynomolgus monkey Tissue lysate - whole (Macaque Retina Lysate)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
200 µg
Specification
Macaque Retina Lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
21 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
24 hour(s), 0 minute(s) and 0 second(s)

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Submitted Aug 15 2016

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