Western blot abreview for Anti-IL-1 beta antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human monocyte, adipose drived stem cells)
Gel Running Conditions
Reduced Denaturing (10% SDS page gel)
Loading amount
30 µg
Treatment
1uM LPS for 24hr
Specification
Human monocyte, adipose drived stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in Skim milk(in PBST)
Dilution
1/1000

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG, HRP-linked Antibody (cell signali
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL™ Select Western Blotting Detection R
Exposure
10 second(s)
Bands
Specific: 40 kDa Non-specific: 55, 70, 170 kDa

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Submitted Sep 18 2018

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