Key features and details
- Rabbit polyclonal to IL-1 beta
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IL-1 beta antibody
See all IL-1 beta primary antibodies
DescriptionRabbit polyclonal to IL-1 beta
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human IL-1 beta (internal sequence). The exact sequence is proprietary.
Database link: P01584
- WB: THP-1 treated with LPS and ATP, whole cell extract. IHC-P: Human lung cancer tissue. IF: THP-1 treated with PMA and LPS, whole cell.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 31 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionPotent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
Tissue specificityExpressed in activated monocytes/macrophages (at protein level).
Sequence similaritiesBelongs to the IL-1 family.
modificationsActivation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
Cellular localizationCytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
- Information by UniProt
- Catabolin antibody
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All lanes : Anti-IL-1 beta antibody (ab226918) at 1/500 dilution
Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell extract at 30 µg
Lane 2 : THP-1 (human monocytic leukemia cell line) treated with 500 ng/ml LPS for 4 hours and 1 mM ATP for 1.5 hours, whole cell extract at 30 µg
Lane 3 : Conditioned medium
Predicted band size: 31 kDa
12% SDS-PAGE gel.
Paraffin-embedded human lung cancer tissue stained for IL-1 beta using ab226918 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunofluorescence analysis of THP-1 (human monocytic leukemia cell line) whole cells (Mock - Left or Treated - Right side) labelling IL-1 beta (green) using ab226918 (Top squares) at 1/500 dilution. Down squares: co-stained with phalloidin (red), a cytoskeleton marker, diluted at 1:100 and fluoroshield with DAPI (blue). Cells treated with 100 ng/ml PMA for 16 hours and 500 ng/ml LPS for 2 hours.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226918 has been referenced in 2 publications.
- Wang L et al. The Effect of Overexpression of the Enhancer of Zeste Homolog 1 (EZH1) Gene on Aristolochic Acid-Induced Injury in HK-2 Human Kidney Proximal Tubule Cells In Vitro. Med Sci Monit 25:801-810 (2019). PubMed: 30688289
- Zhu H et al. TREM-1 deficiency attenuates the inflammatory responses in LPS-induced murine endometritis. Microb Biotechnol N/A:N/A (2019). PubMed: 31365951