Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-1 beta antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (bone marrow-derived macrophages)
Permeabilization
Yes - .01% Triton X100
Specification
bone marrow-derived macrophages
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1% BSA/5% goat serum in PBS
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 586
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568

Additional data

Additional Notes
Cells are immortalised murine bone marrow-derived macrophages stably transfected with GFP-LC3 (green) to visualise autophagosomes.
PubMed ID
21228274: View

Dr. James Harris

Verified customer

Submitted Jan 28 2011

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