Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-1 beta antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (RAW264.7)
Permeabilization
Yes - 0.1% Triton + 2% BSA in PBS
Specification
RAW264.7
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: A488 conjugated Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

PubMed ID
GR1134-3: View

Dr. Mahesh Shivananjappa

Verified customer

Submitted Apr 12 2013

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